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一种易于操作的半自动方法，用于使用比色法活力测定和分数析因设计进行培养基开发。

An easy-to-handle semi-automated method for media development using a colorimetric viability assay and fractional factorial designs.

作者信息

Rexen P, Kierulff J V, Emborg C

机构信息

Department of Biotechnology, Technical University of Denmark, Lyngby.

出版信息

Cytotechnology. 1992;8(3):195-205. doi: 10.1007/BF02522036.

DOI:10.1007/BF02522036
PMID:1368816
Abstract

A rapid and effective semi-automated screening method has been developed for the development of growth media for mammalian cell culture. The method has proven to be a powerful tool for preliminary evaluation and comparison of new media formulations, but has some inherent disadvantages, which are important to recognise in order to interpret the results.

摘要

已开发出一种快速有效的半自动筛选方法，用于开发哺乳动物细胞培养的生长培养基。该方法已被证明是一种用于初步评估和比较新培养基配方的强大工具，但也存在一些固有缺点，为了解释结果，认识到这些缺点很重要。

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本文引用的文献

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Agitation and aeration effects in suspension mammalian cell cultures.悬浮哺乳动物细胞培养中的搅拌和气升效应。
Cytotechnology. 1989 Aug;2(3):163-70. doi: 10.1007/BF00133241.
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Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays.用于细胞生长和存活的快速比色测定法：应用于增殖和细胞毒性测定。
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Use of MTT colorimetric assay to measure cell activation.使用MTT比色法测量细胞活性。
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A study of some variables in a tetrazolium dye (MTT) based assay for cell growth and chemosensitivity.一项基于四唑盐染料（MTT）的细胞生长和化学敏感性检测中某些变量的研究。
Br J Cancer. 1987 Sep;56(3):279-85. doi: 10.1038/bjc.1987.190.
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Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability.用于细胞生长和存活的快速比色测定法。对四氮唑染料程序的改进提高了灵敏度和可靠性。
J Immunol Methods. 1986 May 22;89(2):271-7. doi: 10.1016/0022-1759(86)90368-6.
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Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay.使用微量培养四唑盐分析法，通过人肿瘤细胞系面板进行药物筛选的可行性。
Cancer Res. 1988 Feb 1;48(3):589-601.
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Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell kill.一种用于测量细胞生长/细胞杀伤的精确快速染色方法的重新审视与进一步发展。
J Immunol Methods. 1989 May 12;119(2):203-10. doi: 10.1016/0022-1759(89)90397-9.
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Hybridoma growth and monoclonal antibody production in iron-rich protein-free medium: effect of nutrient concentration.富铁无蛋白培养基中杂交瘤细胞的生长及单克隆抗体的产生：营养物浓度的影响
Cytotechnology. 1991 Sep;7(1):33-8. doi: 10.1007/BF00135636.
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Tetrazolium salts and formazans.四唑盐和甲臜
Prog Histochem Cytochem. 1976;9(3):1-56. doi: 10.1016/s0079-6336(76)80015-0.