Department of Pathology, University of Maryland School of Medicine, 21201, Baltimore, Maryland, USA.
Cytotechnology. 1989 Aug;2(3):187-201. doi: 10.1007/BF00133244.
Malignant A-549 lung carcinoma and adenovirus-12 SV40 hybrid virus transformed non-tumorigenic human bronchial epithelial cells (BEAS-2B) were objectively discriminated from normal bronchial epithelial (BE) cells on the basis of Papanicolaou stained nuclear features (e.g. shape, chromatin texture, hyperchromasia) and nucleolar morphology (e.g. number per cell, irregular contours). Morphometric analysis indicated that significant differences in cellular morphology existed between BE, BEAS-2B, and A-549 cells. Similar analyses of transformed, tumorigenic cell lines demonstrated that nuclear features (i.e., chromatin texture, clearing of parachromatin, hyperchromasia, variation in thickness of the nuclear envelope, sharp indentations in the nuclear envelope), and nucleolar features (i.e., degree of roundness, presence of angular projections, number per cell) discriminated chemically and virally transformed cells from spontaneously transformed cells. Nuclear and nucleolar features were correlated with the growth rate of tumorigenic cell lines. These analytical approaches will be helpful in studies of the effects of various factors (e.g. vitamin A, phorbol ester, oncogene transfection) on cellular proliferation and/or differentiation.
基于巴氏染色核特征（如形状、染色质纹理、异染色质）和核仁形态（如细胞内数量、不规则轮廓），客观地区分了恶性 A-549 肺癌细胞和腺病毒-12 SV40 杂交病毒转化的非致瘤性人支气管上皮细胞（BEAS-2B）与正常支气管上皮（BE）细胞。形态计量分析表明，BE、BEAS-2B 和 A-549 细胞之间存在显著的细胞形态差异。对转化、致瘤细胞系的类似分析表明，核特征（即染色质纹理、副染色质清除、异染色质、核膜厚度变化、核膜的明显凹陷）和核仁特征（即圆度程度、存在角状突起、细胞内数量）区分了化学转化和病毒转化细胞与自发转化细胞。核和核仁特征与致瘤细胞系的生长速率相关。这些分析方法将有助于研究各种因素（如维生素 A、佛波酯、癌基因转染）对细胞增殖和/或分化的影响。
