从用 NS-1 骨髓瘤建立的杂交瘤中删除 κ 基因。
Deletion of the κ genes from mouse hybridomas established with NS-1 myelomas.
出版信息
Cytotechnology. 1997 Sep;24(3):213-8. doi: 10.1023/A:1007910831550.
Monoclonal antibodies (mAbs) of the IgG class produced by mouse hybridomas raised with NS-1 myelomas have been shown to contain two types of immunoglobulin light (κ) chains derived from the myelomas and antigen-stimulated spleen lymphocytes, and the hybridomas produce three mAb species with light chain heterogeneity (Abe and Inouye, 1993). In the present study, 9 hybridoma lines secreting homogeneous mAbs have been isolated from 63 lines cloned from an established hybridoma line producing three mAbs. They secrete homogeneous mAbs containing light chains derived from either myeloma or spleen cells. They contain either κ gene derived from the respective cells, and the other gene was deleted during the cultivation. The deletion frequency of the κ gene of myelomas is 3 times higher than that of spleen cells, although 80-85% of hybridomas reach the stable state containing both κ genes.
用 NS-1 骨髓瘤制备的小鼠杂交瘤产生的 IgG 类单克隆抗体（mAbs）已被证明含有两种免疫球蛋白轻（κ）链，分别来源于骨髓瘤和抗原刺激的脾淋巴细胞，并且杂交瘤产生三种具有轻链异质性的 mAb 种类（Abe 和 Inouye，1993）。在本研究中，从 63 个从产生三种 mAb 的已建立的杂交瘤系克隆的杂交瘤系中分离出 9 个分泌同质 mAb 的杂交瘤系。它们分泌含有源自骨髓瘤或脾细胞的轻链的同质 mAb。它们要么含有源自相应细胞的 κ 基因，要么在培养过程中缺失了另一个基因。骨髓瘤 κ 基因的缺失频率是脾细胞的 3 倍，尽管 80-85%的杂交瘤达到了含有两个 κ 基因的稳定状态。
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