Department of Chemical Engineering, The Ohio State University, 43210-1180, Columbus, OH, USA.
Cytotechnology. 1996 Jan;22(1-3):95-102. doi: 10.1007/BF00353928.
Three dimensional particle tracking velocimetry (3-D PTV) was used to characterize the flow fields in the impeller region of three microcarrier reactor vessels. Three typical cell culture bioreactors were chosen: 250 ml small-scale spinner vessels, 3 L bench-scale reactor, and 20 L medium-scale reactor. Conditions studied correspond to the actual operating conditions in industrial setting and were determined based on the current scale-up paradigm: the Kolmogorov eddy length criterion. In this paper we present characterization of hydrodynamics on the basis of flow structures produced because of agitation. Flow structures were determined from 3-D mean velocity results obtained using 3-D PTV. Although the impellers used in 3 L and 20 L reactors were almost identical, the flow structures produced in the two reactors differed considerably. Results indicate that near geometric scale up does not necessarily amount to scale-up of flow patterns and indicates that intensity as well as distribution of energy may vary considerably during such a scale-up.
三维粒子跟踪测速技术（3-D PTV）用于描述三种微载体反应器中叶轮区域的流场。选择了三种典型的细胞培养生物反应器：250ml 小型搅拌罐、3L 台式反应器和 20L 中型反应器。研究条件对应于工业环境中的实际操作条件，并基于当前的放大范例确定：柯尔莫哥洛夫涡旋长度准则。本文基于搅拌产生的流场结构，对流体动力学进行了描述。流场结构是根据使用 3-D PTV 获得的 3-D 平均速度结果确定的。尽管 3L 和 20L 反应器中使用的叶轮几乎相同，但在两个反应器中产生的流场结构却有很大的不同。结果表明，几何相似放大不一定等同于流型放大，并且在这种放大过程中，能量的强度和分布可能会有很大的变化。
