Papoutsakis E T
Department of Chemical Engineering, Northwestern University, Evanston, IL 60208-3120.
Trends Biotechnol. 1991 Dec;9(12):427-37. doi: 10.1016/0167-7799(91)90145-8.
The fluid-mechanical and some biological aspects of damage to animal cells in bioreactors due to agitation and/or aeration are attracting renewed attention. In microcarrier bioreactors, cell damage is due to forces generated by the interaction of microcarrier beads with each other and also with small turbulent eddies. For freely suspended cells grown in mixed bioreactors, cell damage is most frequently due to bubble breakup or fast-draining liquid films around rearranging gas-liquid interfaces.
生物反应器中因搅拌和/或曝气对动物细胞造成损伤的流体力学及一些生物学方面正重新引起关注。在微载体生物反应器中，细胞损伤是由于微载体珠粒相互作用以及与小湍流涡旋相互作用产生的力所致。对于在混合生物反应器中生长的自由悬浮细胞，细胞损伤最常见的原因是气泡破裂或在重新排列的气液界面周围快速排水的液膜。
