CNR Ctr Cytopharmacol, Ctr B. Ceccarelli, Dept Pharmacol School of Medicine, Univ of Milan, Via Vanvitelli 32, 20129, Milano, Italy.
Cytotechnology. 1991 Feb;5(Suppl 1):99-102. doi: 10.1007/BF00736822.
This paper is a short review of the fluorimetric methods used to measure intracellular free Ca++ concetration in living cells. The availability of fluorescent probes has greatly contributed to the understanding of the mechanisms responsible for the cellular homeostasys of this second messenger. Data can be collected from populations of cells by spectrofluorimetry or from small groups or single cells by spectromicroscopy. Finally the fluorescent images can be captured by a high sensitivity camera, digitally processed and convert in Ca++ images of the cell. The technique allows recognition of differences in [Ca++]i transients among adjacent cells in a same field or in different regions of a cell and greatly contributes to the identification of the cellular mechanisms modulating [Ca++]i.
本文是对用于测量活细胞内游离 Ca++浓度的荧光方法的简短综述。荧光探针的可用性极大地促进了对这种第二信使细胞内稳态的机制的理解。可以通过荧光分光光度法从细胞群体中收集数据，也可以通过分光显微镜从小群或单个细胞中收集数据。最后，可以通过高灵敏度相机捕获荧光图像，对其进行数字处理，并将其转换为细胞的 Ca++图像。该技术允许识别同一视野中相邻细胞或细胞不同区域之间的 [Ca++]i 瞬变差异，并极大地促进了调节 [Ca++]i 的细胞机制的识别。
