Fumagalli G, Zacchetti D, Lorenzon P, Grohovaz F
Ctr B. Ceccarelli, Dept Pharmacol, School of Medicine, Univ of Milan, Italy.
Cytotechnology. 1991;5 Suppl 1:99-102.
This paper is a short review of the fluorimetric methods used to measure intracellular free Ca++ concentration in living cells. The availability of fluorescent probes has greatly contributed to the understanding of the mechanisms responsible for the cellular homeostasis of this second messenger. Data can be collected from populations of cells by spectrofluorimetry or from small groups or single cells by spectromicroscopy. Finally the fluorescent images can be captured by a high sensitivity camera, digitally processed and convert in Ca++ images of the cell. The technique allows recognition of differences in [Ca++]i transients among adjacent cells in a same field or in different regions of a cell and greatly contributes to the identification of the cellular mechanisms modulating [Ca++]i.
本文是一篇关于用于测量活细胞内游离钙离子浓度的荧光法的简短综述。荧光探针的出现极大地促进了人们对这种第二信使细胞内稳态相关机制的理解。数据可以通过荧光分光光度法从细胞群体中收集，或者通过光谱显微镜从小细胞群体或单个细胞中收集。最后，可以用高灵敏度相机捕捉荧光图像，进行数字处理并转换为细胞的钙离子图像。该技术能够识别同一视野中相邻细胞之间或细胞不同区域之间钙离子瞬变的差异，对调节细胞内钙离子浓度的细胞机制的识别有很大帮助。
