Istitute of Biochem. and Evol. Genetic of C.N.R., Via Abbiategrasso, 207, 27100, Pavia, Italy.
Cytotechnology. 1991 Feb;5(Suppl 1):132-3. doi: 10.1007/BF00736830.
Interleukin-6 (IL-6) was recently described to support the growth of Epstein-Barr (EBV) transformed lymphocytes. However no effect was reported on cloning of EBV transformed lymphocytes under limiting dilution conditions. In this paper we demonstrate that the Supernatant of Human Endothelial Cells (HECS) contains a factor(s) different from IL-6 which induces high cloning efficiency of EBV transformed cell lines, cultured under limiting dilution conditions. Furthermore HECS is superior to IL-6 in all the passages of EBV lymphocytes transformation.
白细胞介素-6 (IL-6) 最近被描述为支持 EBV 转化淋巴细胞的生长。然而，在有限稀释条件下，克隆 EBV 转化淋巴细胞并没有报道有任何效果。在本文中，我们证明人内皮细胞上清（HECS）中含有一种不同于 IL-6 的因子（s），它在有限稀释条件下培养时，可诱导 EBV 转化细胞系的高克隆效率。此外，HECS 在 EBV 淋巴细胞转化的所有传代中均优于 IL-6。
