Human Genome Sciences, Inc., 9410 Key West Avenue, Rockville, MD, 20850, U.S.A.
Cytotechnology. 1999 Mar;29(2):151-8. doi: 10.1023/A:1008053421462.
A large number of microcarriers are commercially available. The capability of cells to successfully proliferate on microcarriers varies with cell lines and media. Choosing the right microcarrier for a particular cell line is more than a choice of a microcarrier. It is part of an integrated process design. A detailed picture of cell growth and product formation will not only be essential in identifying the kind of microcarrier, but also in determining other parts of the process, such as operation mode and media. Our initial screening on thirteen microcarriers showed that cultures on some microcarriers reached a low cell density but high cell-specific productivity, and high density microcarrier cultures have a low specific productivity. The result is a similar product output per unit volume and time for these two types of cultures. An ideal culture system shall have increased volumetric productivity at elevated cell density. This requires the process goal to be incorporated as early as cell line construction and screening. A high output process can then be realized through high density culture.
大量的微载体可商购获得。细胞在微载体上成功增殖的能力因细胞系和培养基而异。为特定的细胞系选择合适的微载体不仅仅是选择微载体，而是一个集成过程设计的一部分。细胞生长和产物形成的详细情况不仅对于确定微载体的类型至关重要，而且对于确定过程的其他部分（例如操作模式和培养基）也至关重要。我们对 13 种微载体的初步筛选表明，在某些微载体上培养的细胞密度低，但细胞特异性生产率高，而高密度微载体培养的细胞特异性生产率低。结果是这两种类型的培养物每单位体积和时间的产物输出相似。理想的培养系统应在提高细胞密度的同时提高体积生产率。这要求在细胞系构建和筛选阶段尽早将过程目标纳入其中。然后，通过高密度培养可以实现高产率的过程。
