Gebb C, Clark J M, Hirtenstein M D, Lindgren G, Lindskog U, Lundgren B, Vretblad P
Dev Biol Stand. 1981;50:93-102.
As part of an effort to broaden the applicability and efficiency of microcarrier cell culture various alternative new microcarriers were synthesized. The microcarriers were compared as substrates for the growth of several types of cells and with respect to binding of proteins from the culture medium. Cross-linked dextran has been found to be the most suitable material for a microcarrier matrix and was used as the matrix for the new microcarriers. One type of microcarrier was synthesized so that the charges necessary for cell attachment were present only in the surface layer of the microcarrier in the form of N,N,N-trimethyl-2-hydroxyaminopropyl groups. The resulting microcarriers had a very low capacity to bind proteins from the culture medium (e.g. albumin and IgG) and such proteins could be removed from the cultures more efficiently than when using previous microcarriers. A new principle was used for the development of the other type of microcarrier. A surface layer of cross-linked denatured collagen provided the surface for growth of cells. These microcarriers provided a "natural" substrate for cell growth and allowed improved attachment and spreading of cells with epithelial morphology. Harvesting cells from these microcarriers with proteolytic enzymes was more efficient than with previous microcarriers. The two alternative microcarriers should result in improved process efficiency and have a potential value in the preparation of live vaccines.
为了扩大微载体细胞培养的适用性和效率，人们合成了各种新型替代微载体。对这些微载体作为几种细胞类型生长的底物以及与培养基中蛋白质的结合情况进行了比较。已发现交联葡聚糖是微载体基质最合适的材料，并被用作新型微载体的基质。合成了一种微载体，使得细胞附着所需的电荷仅以N,N,N-三甲基-2-羟氨基丙基的形式存在于微载体的表面层。所得微载体结合培养基中蛋白质（如白蛋白和免疫球蛋白）的能力非常低，并且与使用先前的微载体相比，可以更有效地从培养物中去除此类蛋白质。开发另一种微载体采用了新原理。交联变性胶原蛋白的表面层为细胞生长提供了表面。这些微载体为细胞生长提供了“天然”底物，并能改善具有上皮形态的细胞的附着和铺展。用蛋白水解酶从这些微载体上收获细胞比从先前的微载体上收获更有效。这两种替代微载体应能提高工艺效率，并在活疫苗制备中具有潜在价值。
