Department of Chemistry, University at Albany, SUNY, Albany, NY 12222, USA.
J Am Soc Mass Spectrom. 2012 May;23(5):996-9. doi: 10.1007/s13361-012-0353-7. Epub 2012 Feb 23.
The spermicide nonoxynol-9 is actually a complex mixture of dozens of closely related amphiphilic compounds, and the chemical properties of this assortment significantly hamper its characterization by GC-MS. The inability to perform routine GC-MS testing on nonoxynol-9 has limited its evidentiary value in forensic casework, which relies heavily on this technique for analysis. A disturbing trend in sexual assault is the use of condoms by assailants, to avoid leaving behind DNA evidence that can connect a perpetrator to a victim. This observation necessitates the development of alternative methods for the analysis of trace evidence that can show causal links between a victim and a suspect. Detection of lubricants associated with sexual assault is one such way to establish this connection. The development of GC-MS methods that permit facile detection of both nonoxynol-9 alone and nonoxynol-9 extracted from other complex matrices that have potential as trace evidence in sexual assault is reported. A detection limit of 2.14 μg of nonoxynol-9 is demonstrated, and a detailed mass spectral profile that elaborates on what is known of its structure is provided.
杀精剂壬苯醇醚-9 实际上是由几十种密切相关的两亲性化合物组成的复杂混合物，而这种混合物的化学性质严重阻碍了其通过 GC-MS 进行表征。由于无法对壬苯醇醚-9 进行常规的 GC-MS 测试，其在法医工作中的证据价值受到限制，而法医工作严重依赖于这种技术进行分析。性侵犯中一个令人不安的趋势是攻击者使用避孕套，以避免留下可以将犯罪者与受害者联系起来的 DNA 证据。这一观察结果需要开发替代方法来分析微量证据，以显示受害者和嫌疑人之间的因果关系。检测与性侵犯相关的润滑剂就是建立这种联系的一种方法。本文报道了开发 GC-MS 方法的进展，这些方法可以方便地检测单独的壬苯醇醚-9 以及从其他可能作为性侵犯中微量证据的复杂基质中提取的壬苯醇醚-9。证明了壬苯醇醚-9 的检测限为 2.14μg，并提供了详细的质谱谱图，详细说明了其结构的已知信息。
