杀精剂壬苯醇醚-9低聚物成分的高效液相色谱（HPLC）分析

High-performance liquid chromatographic (HPLC) analysis of oligomeric components of the spermicide nonoxynol-9.

作者信息

Walter B A, Digenis G A

机构信息

College of Pharmacy, University of Kentucky, Lexington 40536-0082.

出版信息

Pharm Res. 1991 Mar;8(3):409-11. doi: 10.1023/a:1015870304964.

DOI:10.1023/a:1015870304964

PMID:1647008

Abstract

The commercially available Nonoxynol-9 spermicide is a multicomponent mixture of oligomers. When Nonoxynol-9 was separated by normal phase gradient HPLC, 17 components were shown to exist in the commercial mixture. These oligomeric components follow a Poisson distribution around the most abundant oligomer, EO 8 (11.7%). Select oligomers were isolated by preparative HPLC (Rt = 19.6, 34.0, 45.6, 51.2, 61.6, and 79.2 min) and purified by HPLC. These were identified by FAB-MS and NMR to be the oligomers EO 3, EO 6, EO 8, EO 9, EO 11, and EO 16, respectively.

摘要

市售的壬苯醇醚-9杀精剂是一种低聚物的多组分混合物。当通过正相梯度高效液相色谱法分离壬苯醇醚-9时，商业混合物中显示存在17种成分。这些低聚成分围绕最丰富的低聚物EO 8（11.7%）呈泊松分布。通过制备型高效液相色谱法（保留时间分别为19.6、34.0、45.6、51.2、61.6和79.2分钟）分离出选定的低聚物，并通过高效液相色谱法进行纯化。通过快原子轰击质谱法（FAB-MS）和核磁共振（NMR）鉴定，这些分别为低聚物EO 3、EO 6、EO 8、EO 9、EO 11和EO 16。