Technische Hochschule Darmstadt, Inst. f. Biochemie, Petersenstr. 22, D-64287, Darn stadt, Germany, dh7y@.pop.th-darmstadt.de.
Cytotechnology. 1994 Jan;16(1):43-50. doi: 10.1007/BF00761778.
Cultures of endothelial cells and cell lines of endothelial origin were maintained at confluence without medium exchange for a period of 72 h. During this time period the concentration of nutrients - amino acids and glucose - and metabolic waste products - lactate and ammonium - was determined as well as cell vitality and cell numbers. Metabolic rates were calculated and compared for the different cell lines. Surprisingly the primary cells showed significantly higher rates of glucose and glutamine consumption, respectively lactate production than the immortalized cell lines. Except for one tumorigenic cell line all cells showed a significant participation of transaminases in glutamine/ammonium metabolism. Furthermore it could be shown that in routine culture there was no depletion of nutrients or critical accumulation of ammonium or lactate over a culture period of 72 h.
将内皮细胞和内皮细胞来源的细胞系培养至汇合状态，在不进行培养基更换的情况下维持 72 小时。在此期间，测定了营养物质（氨基酸和葡萄糖）和代谢废物（乳酸盐和铵）的浓度，以及细胞活力和细胞数量。计算并比较了不同细胞系的代谢率。令人惊讶的是，与永生化细胞系相比，原代细胞的葡萄糖和谷氨酰胺摄取率以及乳酸盐生成率分别显著更高。除了一个致瘤细胞系外，所有细胞在谷氨酰胺/铵代谢中均显著参与氨基转移酶。此外，还可以证明在常规培养中，在 72 小时的培养期间，没有营养物质耗尽或铵或乳酸盐的临界积累。
