一种支持人脐静脉内皮细胞长期培养的无血清培养基配方。
A serum-free medium formulation supporting growth of human umbilical cord vein endothelial cells in long-term cultivation.
机构信息
Institut für Biochemie, Technische Universität Darmstadt, Petersenstrasse 22, 64287, Darmstadt, Germany.
出版信息
Cytotechnology. 2001 Mar;35(2):87-92. doi: 10.1023/A:1017551218007.
Abstract
A serum-free medium formulation - TUD-1 - was developed supporting growth of HUVEC in tissue culture. Special features of the basal medium formulation are highly elevated levels of glutamine and serine as well as the inclusion of N-acetylcysteine and phosphoascorbic acid. The cellular mitogenic needs are satisfied by bFGF, VEGF, EGF and liver growth factor. Further hormone supplementation consists of insulin and hydrocortisone. A protocoll for serum-free passage of HUVEC was established for serum-free long-term cultivation of freshly isolated HUVEC for up to 20 cumulative population doublings without significant differences in final cell density compared to controls cultivated with serum.
摘要
开发了一种无血清培养基配方-TUD-1-，可支持组织培养中的 HUVEC 生长。基础培养基配方的特点是谷氨酰胺和丝氨酸含量极高，以及包含 N-乙酰半胱氨酸和磷酸抗坏血酸。细胞有丝分裂的需要通过 bFGF、VEGF、EGF 和肝生长因子来满足。进一步的激素补充包括胰岛素和氢化可的松。建立了 HUVEC 无血清传代的方案，用于无血清长期培养新鲜分离的 HUVEC，可进行多达 20 次累积传代，与用血清培养的对照相比，最终细胞密度没有显著差异。
相似文献
1
A serum-free medium formulation supporting growth of human umbilical cord vein endothelial cells in long-term cultivation.一种支持人脐静脉内皮细胞长期培养的无血清培养基配方。
Cytotechnology. 2001 Mar;35(2):87-92. doi: 10.1023/A:1017551218007.
2
Growth and continuous passage of COMMA-D mouse mammary epithelial cells in hormonally defined serum-free medium.逗号-D小鼠乳腺上皮细胞在激素限定的无血清培养基中的生长及连续传代。
Cancer Res. 1987 Jul 15;47(14):3776-82.
3
[Differential influences of bFGF and VEGF on the expression of vascular cell adhesion molecule-1 on human umbilical vein endothelial cells].[碱性成纤维细胞生长因子和血管内皮生长因子对人脐静脉内皮细胞血管细胞黏附分子-1表达的不同影响]
Nihon Rinsho Meneki Gakkai Kaishi. 2000 Feb;23(1):12-21. doi: 10.2177/jsci.23.12.
4
Growth factors and corneal endothelial cells: I. Stimulation of bovine corneal endothelial cell DNA synthesis by defined growth factors.生长因子与角膜内皮细胞：I.特定生长因子对牛角膜内皮细胞DNA合成的刺激作用
Cornea. 1992 Jan;11(1):1-10. doi: 10.1097/00003226-199201000-00001.
5
Use of a serum-free medium for long-term storage of human corneas. Influence on endothelial cell density and corneal metabolism.使用无血清培养基长期保存人角膜。对内皮细胞密度和角膜代谢的影响。
Graefes Arch Clin Exp Ophthalmol. 2001 Oct;239(10):801-5. doi: 10.1007/s004170100364.
6
7
Long-term serial cultivation and growth requirements for human umbilical vein endothelial cells.人脐静脉内皮细胞的长期连续培养及生长需求
In Vitro Cell Dev Biol. 1985 Mar;21(3 Pt 1):181-8. doi: 10.1007/BF02621356.
8
Growth kinetics and differentiation in vitro of normal human uroepithelial cells on collagen gel substrates in defined medium.正常人尿道上皮细胞在特定培养基中胶原凝胶底物上的体外生长动力学及分化
J Cell Physiol. 1987 Jun;131(3):285-301. doi: 10.1002/jcp.1041310302.
9
[Biological effects of paracrine from insulin stimulated adipose-derived stem cells (ADSC) on human vascular endothelial cells].胰岛素刺激的脂肪来源干细胞（ADSC）旁分泌对人血管内皮细胞的生物学效应
Zhonghua Shao Shang Za Zhi. 2011 Feb;27(1):32-6.
10
Hypoxia stimulates human endothelial cells to release smooth muscle cell mitogens: role of prostaglandins and bFGF.缺氧刺激人内皮细胞释放平滑肌细胞促分裂原：前列腺素和碱性成纤维细胞生长因子的作用。
Exp Cell Res. 1994 Jul;213(1):43-54. doi: 10.1006/excr.1994.1171.
引用本文的文献
1
The Development of 3D Primary Co-Culture Models of the Human Airway.人呼吸道三维原代共培养模型的发展
Int J Mol Sci. 2025 May 23;26(11):5027. doi: 10.3390/ijms26115027.
2
The Potential for Placental Activation of PPARγ to Improve the Angiogenic Profile in Preeclampsia.胎盘激活 PPARγ 改善子痫前期血管生成谱的潜力。
Cells. 2022 Nov 6;11(21):3514. doi: 10.3390/cells11213514.
3
Sex-Specific Response to Combinations of Shear Stress and Substrate Stiffness by Endothelial Cells In Vitro.体外研究内皮细胞对切应力和基质硬度组合的性别特异性反应。
Adv Healthc Mater. 2021 Sep;10(18):e2100735. doi: 10.1002/adhm.202100735. Epub 2021 Jun 17.
4
Recent Expansions on Cellular Models to Uncover the Scientific Barriers Towards Drug Development for Alzheimer's Disease.近期对细胞模型的扩展，以揭示阿尔茨海默病药物开发的科学障碍。
Cell Mol Neurobiol. 2019 Mar;39(2):181-209. doi: 10.1007/s10571-019-00653-z. Epub 2019 Jan 23.
5
Secreted Endothelial Cell Factors Immobilized on Collagen Scaffolds Enhance the Recipient Endothelial Cell Environment.固定在胶原蛋白支架上的分泌型内皮细胞因子可改善受体内皮细胞环境。
Biores Open Access. 2016 Mar 1;5(1):61-71. doi: 10.1089/biores.2016.0003. eCollection 2016.
6
Enhanced neovascular formation in a novel hydrogel matrix consisting of citric Acid and collagen.在由柠檬酸和胶原蛋白组成的新型水凝胶基质中增强的新生血管形成。
Ann Vasc Dis. 2011;4(3):196-203. doi: 10.3400/avd.oa.11.00017. Epub 2011 Jun 2.
本文引用的文献
1
Serum-free growth medium for the cultivation of a wide spectrum of mammalian cells in stirred bioreactors.无血清生长培养基，用于搅拌式生物反应器中培养多种哺乳动物细胞。
Cytotechnology. 1988 Nov;1(4):319-29. doi: 10.1007/BF00365077.
2
Metabolic rates of vascular endothelial cellsin vitro.血管内皮细胞的体外代谢率。
Cytotechnology. 1994 Jan;16(1):43-50. doi: 10.1007/BF00761778.
3
An optimized culture medium for human vascular endothelial cells from umbilical cord veins.人脐带静脉血管内皮细胞的优化培养基。
Cytotechnology. 1989 Aug;2(3):171-9. doi: 10.1007/BF00133242.
4
Growth and physiological functions of vascular endothelial cells in a new serum-free medium (SFM).新型无血清培养基（SFM）中血管内皮细胞的生长及生理功能
Exp Cell Res. 1993 Jun;206(2):291-301. doi: 10.1006/excr.1993.1149.
5
Preservation of differentiated phenotypes in cultured aortic endothelial cells by malotilate and phosphoascorbic acid.马洛替酯和磷酸抗坏血酸对培养的主动脉内皮细胞分化表型的保存作用。
Eur J Cell Biol. 1993 Feb;60(1):48-56.
6
Serial propagation of human endothelial cells in vitro.人内皮细胞在体外的连续传代培养
J Cell Biol. 1981 Nov;91(2 Pt 1):420-6. doi: 10.1083/jcb.91.2.420.
7
Phosphatidyl choline and the growth in serum-free medium of vascular endothelial and smooth muscle cells, and corneal endothelial cells.
J Cell Physiol. 1983 Mar;114(3):267-78. doi: 10.1002/jcp.1041140304.
8
The role of fibroblast growth factor and the extracellular matrix in the control of proliferation and differentiation of corneal endothelial cells.成纤维细胞生长因子和细胞外基质在角膜内皮细胞增殖与分化调控中的作用。
Vision Res. 1981;21(1):87-103. doi: 10.1016/0042-6989(81)90141-3.
9
Brain- and liver cell-derived factors are required for growth of human endothelial cells in serum-free culture.人脑和肝细胞衍生因子是无血清培养中人类内皮细胞生长所必需的。
Proc Natl Acad Sci U S A. 1984 Oct;81(20):6413-7. doi: 10.1073/pnas.81.20.6413.
10
Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria.源自脐静脉的人内皮细胞培养。通过形态学和免疫学标准进行鉴定。
J Clin Invest. 1973 Nov;52(11):2745-56. doi: 10.1172/JCI107470.
Zotero 插件