Institut für Biochemie, Technische Universität Darmstadt, Petersenstrasse 22, 64287, Darmstadt, Germany.
Cytotechnology. 2001 Mar;35(2):87-92. doi: 10.1023/A:1017551218007.
A serum-free medium formulation - TUD-1 - was developed supporting growth of HUVEC in tissue culture. Special features of the basal medium formulation are highly elevated levels of glutamine and serine as well as the inclusion of N-acetylcysteine and phosphoascorbic acid. The cellular mitogenic needs are satisfied by bFGF, VEGF, EGF and liver growth factor. Further hormone supplementation consists of insulin and hydrocortisone. A protocoll for serum-free passage of HUVEC was established for serum-free long-term cultivation of freshly isolated HUVEC for up to 20 cumulative population doublings without significant differences in final cell density compared to controls cultivated with serum.
开发了一种无血清培养基配方-TUD-1-，可支持组织培养中的 HUVEC 生长。基础培养基配方的特点是谷氨酰胺和丝氨酸含量极高，以及包含 N-乙酰半胱氨酸和磷酸抗坏血酸。细胞有丝分裂的需要通过 bFGF、VEGF、EGF 和肝生长因子来满足。进一步的激素补充包括胰岛素和氢化可的松。建立了 HUVEC 无血清传代的方案，用于无血清长期培养新鲜分离的 HUVEC，可进行多达 20 次累积传代，与用血清培养的对照相比，最终细胞密度没有显著差异。
