Sun Yuan, Sriramajayam Kannappan, Luo Dianzhong, Liao D Joshua
1. Hormel Institute, the University of Minnesota, Austin, MN 55912, USA.
J Cancer. 2012;3:93-5. doi: 10.7150/jca.4163. Epub 2012 Feb 13.
In this short communication we report a quick, cost-free method of purification of DNA fragments from agarose gel. Unlike those procedures that involve commercial kits, this method uses glass wool or absorbent cotton to filter agarose gel during a quick spinning-down of DNA, thus significantly simplifying the routine practice of many molecular biologists and decreasing the cost.
在本简短通讯中,我们报告了一种从琼脂糖凝胶中快速、免费纯化DNA片段的方法。与那些涉及商业试剂盒的程序不同,该方法在快速沉淀DNA的过程中使用玻璃棉或脱脂棉过滤琼脂糖凝胶,从而显著简化了许多分子生物学家的常规操作并降低了成本。