World Vegetable Center, 60 Yi-Min Liao, Shanhua, 74151, Tainan, Taiwan.
World Vegetable Center, East and Southeast Asia, Kasetsart University, Kamphaeng Saen, Nakhon Pathom, 73140, Thailand.
Sci Rep. 2024 Mar 21;14(1):6793. doi: 10.1038/s41598-024-57348-9.
Virus diseases are a major production constraint for pumpkin. Recessive resistance to squash leaf curl China virus and tomato leaf curl New Delhi virus has been mapped in Cucurbita moschata (Duchesne ex Poir.) breeding line AVPU1426 to chromosomes 7 and 8, respectively. Molecular markers tightly associated with the resistance loci have been developed and were able to correctly predict resistance and susceptibility with an accuracy of 99% for squash leaf curl China virus resistance and 94.34% for tomato leaf curl New Delhi virus in F and back cross populations derived from the original resistance source AVPU1426. The markers associated with resistance are recommended for use in marker-assisted breeding.
病毒病是南瓜生产的主要限制因素。在南瓜品种 AVPU1426 中，已经定位到了对南瓜曲叶中国病毒和番茄曲叶新德里病毒的隐性抗性，分别位于第 7 号和第 8 号染色体上。已经开发出与抗性基因紧密连锁的分子标记，可以准确预测抗性和敏感性，对南瓜曲叶中国病毒的抗性预测准确率为 99%，对番茄曲叶新德里病毒的抗性预测准确率为 94.34%，这些预测结果在源自原始抗性源 AVPU1426 的 F2 和回交群体中得到了验证。建议使用与抗性相关的标记进行标记辅助选择育种。
