Aalto University, School of Chemical Technology, Department of Forest Products Technology, Espoo, Finland.
Biomacromolecules. 2012 Apr 9;13(4):1051-8. doi: 10.1021/bm201771m. Epub 2012 Mar 13.
In this Article, we present a new strategy for preparing an antihemoglobin biointerface on cellulose. The preparation method is based on functionalization of the cellulose surface by the irreversible adsorption of CMC, followed by covalent linking of antibodies to CMC. This would provide the means for affordable and stable cellulose-based biointerfaces for immunoassays. The preparation and characterization of the biointerface were studied on Langmuir-Schaefer cellulose model surfaces in real time using the quartz crystal microbalance with dissipation and surface plasmon resonance techniques. The stable attachment of antihemoglobin to adsorbed CMC was achieved, and a linear calibration of hemoglobin was obtained. CMC modification was also observed to prevent nonspecific protein adsorption. The antihemoglobin-CMC surface regenerated well, enabling repeated immunodetection cycles of hemoglobin on the same surface.
在本文中，我们提出了一种在纤维素上制备抗血红蛋白生物界面的新策略。该制备方法基于通过 CMC 的不可逆吸附对纤维素表面进行功能化，然后将抗体共价连接到 CMC 上。这将为免疫分析提供经济实惠且稳定的基于纤维素的生物界面。使用石英晶体微天平实时研究了生物界面的制备和表征，该技术结合了耗散和表面等离子体共振技术。成功实现了抗血红蛋白与吸附 CMC 的稳定附着，并获得了血红蛋白的线性校准。还观察到 CMC 修饰可以防止非特异性蛋白质吸附。抗血红蛋白-CMC 表面再生良好，使同一表面上的血红蛋白可以进行多次免疫检测循环。
