Seto Hirokazu, Kamba Seiji, Kondo Takashi, Ogawa Yuichi, Hoshino Yu, Miura Yoshiko
Graduate School of Engineering, Kyushu University.
Anal Sci. 2015;31(3):173-6. doi: 10.2116/analsci.31.173.
A biosensor for protein detection was developed using antibody-immobilized metal mesh devices. Antihemoglobin antibodies were covalently immobilized on a metal mesh device. Extraordinary transmission with a dipped structure was observed for a metal mesh device immobilized with antihemoglobin antibodies as well as for the original metal mesh device. Hemoglobin in the mixture solution containing albumin at a hundred-fold concentration was detectable using antihemoglobin-immobilized MMDs. The detectability using the antihemoglobin-immobilized metal mesh device was similar to that of a commercially-available kit for the qualitative determination of hemoglobin.
利用抗体固定化金属网装置开发了一种用于蛋白质检测的生物传感器。抗血红蛋白抗体被共价固定在金属网装置上。对于固定有抗血红蛋白抗体的金属网装置以及原始金属网装置，均观察到具有浸入结构的超常透射现象。使用固定有抗血红蛋白的金属微器件（MMDs）可检测含有浓度高一百倍白蛋白的混合溶液中的血红蛋白。使用固定有抗血红蛋白的金属网装置的检测能力与用于血红蛋白定性测定的市售试剂盒相似。
