Department of Food Science and Nutrition, Graduate School of Venture for BK21, Hoseo University, Asan, South Korea.
J Agric Food Chem. 2012 Mar 14;60(10):2517-24. doi: 10.1021/jf205086b. Epub 2012 Mar 6.
Diabetes mellitus, which is associated with oxidative damage, has a significant impact on health, quality of life, and life expectancy. An ethanol extract of Gymnema sylvestre leaf was examined in vitro and in vivo to investigate the role of antioxidants in diabetic rats. The extract exhibited strong antioxidant activity in the assays, including TBA (56%), SOD-like (92%), and ABTS (54%). Blood glucose levels in the diabetic rats fed G. sylvestre extract decreased to normal levels. The presence of the antihyperglycemic compounds gymnemagenin and gymnemic acids in G. sylvestre extract was detected by LC/MS analysis. Lipid peroxidation levels were decreased by 31.7% in serum, 9.9% in liver, and 9.1% in kidney in the diabetic rats fed the extract. Feeding G. sylvestre extract to the diabetic rats decreased the activity of glutathione peroxidase in cytosolic liver and glutamate pyruvate transaminase in serum to normal levels.
糖尿病与氧化损伤有关，对健康、生活质量和预期寿命有重大影响。我们研究了匙羹藤叶的乙醇提取物在糖尿病大鼠体内和体外的抗氧化作用。该提取物在 TBA（56%）、SOD 样（92%）和 ABTS（54%）测定中表现出很强的抗氧化活性。喂食匙羹藤提取物的糖尿病大鼠的血糖水平降至正常水平。通过 LC/MS 分析检测到匙羹藤提取物中存在降血糖化合物甘木聚糖和匙羹藤酸。提取物可使血清中脂质过氧化水平降低 31.7%，肝脏中降低 9.9%，肾脏中降低 9.1%。喂食匙羹藤提取物可使糖尿病大鼠肝脏胞质中的谷胱甘肽过氧化物酶和血清中的谷氨酸丙酮酸转氨酶的活性恢复正常。
