Department of Chemistry & Institute of Biomedical Science & State Key Laboratory of Molecular Engineering of Polymers, Fudan University, Shangai 200433, PR China.
Biomaterials. 2012 May;33(14):3733-42. doi: 10.1016/j.biomaterials.2012.01.063. Epub 2012 Feb 21.
A new upconversion luminescence (UCL) nanophosphors based on host matrix of cubic NaLuF(4) with bright luminescence have been synthesized by a solvothermal method, facilitate the nanocrystals potential candidates for imaging in vivo, especially large-animals. The sub-20 nm NaLuF(4) co-doped Yb(3+) and Er(3+) (Tm(3+)) showed about 10-fold stronger UCL emission than that of corresponding hexagonal NaYF(4)-based nanocrystals with a 20 nm diameter. Near-infrared to near-infrared (NIR-to-NIR) UCL emission of PAA-coated NaLuF(4):20%Yb,1%Tm (PAA-Lu(Tm)) can penetrate >1.5 cm tissue of pork with high contrast. Based on super-strong UCL emission and deep penetration, PAA-Lu(Tm) as optical bioprobe has been demonstrated by in vivo UCL imaging of a normal black mouse, even rabbit with excellent signal-to-noise ratio. Furthermore, such cubic NaLuF(4)-based nanophosphor was applied in lymph node imaging of live Kunming mouse with rich white fur.
一种基于立方 NaLuF(4)基质的新型上转换发光（UCL）纳米荧光粉，具有明亮的发光性能，通过溶剂热法合成，为体内成像，特别是大动物成像提供了潜在的纳米晶候选物。亚 20nm 的 NaLuF(4)共掺 Yb(3+)和 Er(3+)（Tm(3+)）的 UCL 发射强度比相应的直径为 20nm 的六方 NaYF(4)基纳米晶强约 10 倍。经聚丙烯酸（PAA）包覆的 NaLuF(4):20%Yb,1%Tm（PAA-Lu(Tm)）具有近红外到近红外（NIR-to-NIR）UCL 发射，可在猪肉组织中穿透 >1.5cm 仍保持高对比度。基于超强 UCL 发射和深穿透，PAA-Lu(Tm)作为光学生物探针，在正常黑鼠和具有良好信噪比的兔子的体内 UCL 成像中得到了证明。此外，这种基于立方 NaLuF(4)的纳米荧光粉还应用于活昆明小白鼠的淋巴结成像，其具有丰富的白色皮毛。
