Abolhassani M, Riley W M, Feldman E, Arlin Z, Chiao J W
Department of Medicine, New York Medical College, Valhalla 10595.
Proc Soc Exp Biol Med. 1990 Nov;195(2):288-91. doi: 10.3181/00379727-217-2-rc1.
Conditioned medium from mitogen stimulated normal peripheral blood lymphocytes (PBL) has been demonstrated to contain a maturation inducer activity mediating the differentiation of human myeloid leukemia cells to monocytes and macrophages. The maturation inducer activity was isolated by salt precipitation, Sepharose CL-6B ion exchange and affinity chromatographies and electrophoresis. Two separate activities with M.W. ranges of 52-56 and 32-35 kDa capable of mediating the terminal differentiation of leukemic HL-60 promyelocytes to monocytes and macrophages were detected. The higher molecular weight species was determined to be a 54 kDa single polypeptide and was found to be distinct from IL-3 and IL-6 by ELISA and differentiation blocking assay. The inducing activity of the 32-35 kDa material was largely neutralized after treatment with anti-IL-3, but not with other antibodies. Employing the immunofluorescent antibody technique, the 54 kDa protein was detected on the surface membranes of PBL. The proportions and number of maturation inducer bearing lymphocytes in patients with acute myelogenous leukemia (0.4% and 35/mm3, respectively) were significantly lower than that of healthy donors (7.9% and 178/mm3) The role of these physiological factors in leukemia cell differentiation is discussed.
有丝分裂原刺激的正常外周血淋巴细胞（PBL）的条件培养基已被证明含有一种成熟诱导活性，可介导人类髓系白血病细胞向单核细胞和巨噬细胞分化。通过盐沉淀、琼脂糖CL-6B离子交换、亲和层析和电泳分离出成熟诱导活性。检测到两种分子量范围分别为52-56 kDa和32-35 kDa的独立活性，它们能够介导白血病HL-60早幼粒细胞向单核细胞和巨噬细胞的终末分化。较高分子量的物质被确定为一种54 kDa的单一多肽，通过ELISA和分化阻断试验发现它与IL-3和IL-6不同。用抗IL-3处理后，32-35 kDa物质的诱导活性大部分被中和，但用其他抗体处理则不然。采用免疫荧光抗体技术，在PBL的表面膜上检测到了54 kDa的蛋白质。急性髓性白血病患者中携带成熟诱导物的淋巴细胞比例和数量（分别为0.4%和35/mm³）显著低于健康供体（7.9%和178/mm³）。本文讨论了这些生理因素在白血病细胞分化中的作用。
