Hosoda Akifumi, Takahashi Tomonori, Numano Kousuke, Nakajou Kunihide, Higashimoto Asumi, Toda Minoru, Arai Hidehiko, Hotta Yudai, Tamura Hiroto
Department of Environmental Bioscience, Meijo University.
J Oleo Sci. 2012;61(3):155-61. doi: 10.5650/jos.61.155.
A case study of the bioremediation of groundwater contaminated with trichloroethene (TCE) was conducted using the biostimulation agent, BD-1. TCE levels were monitored by gas chromatography-mass spectroscopy. Total organic carbon (TOC) and volatile fatty acids (VFAs) were analyzed to investigate the environmental fate of BD-1. The effects of BD-1 on microbial activity were investigated using 16S rRNA gene-based quantitative polymerase chain reaction (qPCR) analysis. The biodegradation of BD-1 was accompanied by a reduction in TCE, and the initially high TOC levels decreased rapidly as BD-1 was transformed into VFAs. qPCR analysis showed that the genus Dehalobacter became progressively dominant through the experiment. These results suggested that BD-1 might dechlorinate TCE by activating dechlorinating bacteria.
使用生物刺激剂BD-1对受三氯乙烯（TCE）污染的地下水进行了生物修复的案例研究。通过气相色谱-质谱法监测TCE水平。分析了总有机碳（TOC）和挥发性脂肪酸（VFA）以研究BD-1的环境归宿。使用基于16S rRNA基因的定量聚合酶链反应（qPCR）分析研究了BD-1对微生物活性的影响。BD-1的生物降解伴随着TCE的减少，并且随着BD-1转化为VFA，最初较高的TOC水平迅速下降。qPCR分析表明，在整个实验过程中，脱卤杆菌属逐渐占主导地位。这些结果表明，BD-1可能通过激活脱氯细菌使TCE脱氯。
