BioAnalytical and Nanobiomedicinal Laboratory, Department of Biochemical Science and Technology, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 10617 Taiwan.
Anal Chem. 2012 Apr 3;84(7):3246-53. doi: 10.1021/ac203362g. Epub 2012 Mar 14.
In this paper, we describe a simple one-pot method, employing l-3,4-dihydroxyphenylalanine (L-DOPA) as a reducing/capping reagent, for the synthesis of fluorescent gold nanoclusters (AuNCs). Within a short reaction time of 15 min (excluding the time required for purification), this strategy allows the fabrication of homogeneous AuNCs having the capability to sense ferric ions (Fe(3+)). The as-prepared AuNCs exhibited a fluorescence emission at 525 nm and a quantum yield of 1.7%. On the basis of an aggregation-induced fluorescence quenching mechanism, these fluorescent AuNCs offer acceptable sensitivity, high selectivity, and a limit of detection of 3.5 μM for the determination of Fe(3+) ions, which is lower than the maximum level (0.3 mg L(-1), equivalent to 5.4 μM) of Fe(3+) permitted in drinking water by the U.S. Environmental Protection Agency.
本文描述了一种简单的一锅法，采用 l-3,4-二羟基苯丙氨酸（L-DOPA）作为还原剂/封端剂，用于合成荧光金纳米簇（AuNCs）。在 15 分钟的短反应时间内（不包括纯化所需的时间），该策略可以制备具有检测三价铁离子（Fe(3+)）能力的均一金纳米簇。所制备的 AuNCs 在 525nm 处表现出荧光发射，量子产率为 1.7%。基于聚集诱导荧光猝灭机制，这些荧光 AuNCs 对 Fe(3+)离子的检测具有可接受的灵敏度、高选择性和检测限为 3.5 μM，低于美国环境保护署规定的饮用水中允许的 Fe(3+)最大水平（0.3 毫克/升，相当于 5.4 μM）。
