MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, China.
Colloids Surf B Biointerfaces. 2012 Jun 1;94:22-6. doi: 10.1016/j.colsurfb.2012.01.006. Epub 2012 Jan 24.
Zirconium-phosphonate (Zr-P) ionic complexation chemistry is explored as a new approach to fabricate poly[2-(methacryloyloxy) ethyl phosphorylcholine] (PMPC) multilayer film by layer-by-layer self-assembly method. Quartz crystal microbalance with dissipation (QCM-D) and optical ellipsometry measurements demonstrated that PMPC layer can be fully absorbed on each Zr(4+) layer. The thickness of the multilayer film with a good linear relationship was followed by the ellipsometry in situ adlayer characterization. The influence of pH of the PMPC and Zr(4+) solutions on the multilayer deposition were investigated by optical ellipsometry. QCM-D results indicated that the multilayer film is stable in a PBS flowing chamber at a high flow rate of 5.2×10(-3)m/s. The ellipsometry data demonstrated that 67.2% of the film still remained on the silicon wafer after being strong shaken in PBS at 80 rpm for 12h. The adsorption of bovine serum albumin (BSA) and fetal bovine serum (FBS) on the PMPC surface was monitored by the QCM-D and spectroscopic ellipsometry, and the results showed the multilayer film have excellent protein resistance.
锆-膦酸盐（Zr-P）离子络合化学被探索作为一种新方法，通过层层自组装方法来制备聚[2-（甲基丙烯酰氧基）乙基磷酸胆碱]（PMPC）多层膜。石英晶体微天平（QCM-D）和椭圆光度法测量表明，PMPC 层可以完全被每个 Zr（4+）层吸收。通过原位吸附加层表征的椭圆光度法跟踪了具有良好线性关系的多层膜的厚度。通过椭圆光度法研究了 PMPC 和 Zr（4+）溶液的 pH 值对多层沉积的影响。QCM-D 结果表明，在高流速 5.2×10（-3）m/s 的 PBS 流动室内，多层膜稳定。椭圆光度法数据表明，在 PBS 中以 80rpm 强烈摇动 12h 后，仍有 67.2%的薄膜留在硅片上。通过 QCM-D 和光谱椭圆光度法监测了牛血清白蛋白（BSA）和胎牛血清（FBS）在 PMPC 表面上的吸附，结果表明该多层膜具有优异的抗蛋白质性。
