Rana R, Vitale M, Mazzotti G, Manzoli L, Papa S
Istituto di Anatomia Umana Normale, Università di Bologna, Italy.
Radiat Res. 1990 Oct;124(1):96-102.
The sensitivity of human natural killer (NK) cell activities (both binding and killing) after exposure of peripheral blood mononuclear cells to different doses of gamma radiation was studied. A panel of monoclonal antibodies was used to identify the NK and T-lymphocyte subsets and to evaluate their radiosensitivity. Peripheral blood mononuclear cells were irradiated with low (2-6 Gy) and high (10-30 Gy) doses and NK cell binding and cytotoxic activity against K562 target cells were studied after 3 h and 48 h in culture. The primary damage to NK cell activity was identified at the postbinding level and affected mainly the lytic machinery. After 48 h culture postirradiation, an overall depression of cytotoxic activity was observed, but ionizing radiation produced either a selection of the more cytotoxic NK cell subsets, which therefore might be considered more resistant to radiation damage than the less cytotoxic NK cells, or a long-term stimulation of cytotoxic activity in surviving cells.
研究了外周血单个核细胞暴露于不同剂量γ射线后人类自然杀伤（NK）细胞活性（结合和杀伤）的敏感性。使用一组单克隆抗体来鉴定NK和T淋巴细胞亚群，并评估它们的放射敏感性。外周血单个核细胞接受低剂量（2 - 6 Gy）和高剂量（10 - 30 Gy）照射，培养3小时和48小时后研究NK细胞对K562靶细胞的结合和细胞毒性活性。NK细胞活性的主要损伤在结合后水平被识别，并且主要影响溶解机制。照射后48小时培养后，观察到细胞毒性活性总体下降，但电离辐射要么导致选择出细胞毒性更强的NK细胞亚群，因此这些亚群可能被认为比细胞毒性较弱的NK细胞对辐射损伤更具抗性，要么导致存活细胞的细胞毒性活性受到长期刺激。
