Tian Yingfang, Stamova Boryana, Jickling Glen C, Xu Huichun, Liu Dazhi, Ander Bradley P, Bushnell Cheryl, Zhan Xinhua, Turner Renee J, Davis Ryan R, Verro Piero, Pevec William C, Hedayati Nasim, Dawson David L, Khoury Jane, Jauch Edward C, Pancioli Arthur, Broderick Joseph P, Sharp Frank R
Department of Neurology and the MIND Institute, University of California at Davis, Sacramento, California 95817, USA.
Gend Med. 2012 Apr;9(2):68-75.e3. doi: 10.1016/j.genm.2012.01.005. Epub 2012 Feb 24.
Sex is suggested to be an important determinant of ischemic stroke risk factors, etiology, and outcome. However, the basis for this remains unclear. The Y chromosome is unique in males. Genes expressed in males on the Y chromosome that are associated with stroke may be important genetic contributors to the unique features of males with ischemic stroke, which would be helpful for explaining sex differences observed between men and women.
We compared Y chromosome gene expression in males with ischemic stroke and male controls.
Blood samples were obtained from 40 male patients ≤3, 5, and 24 hours after ischemic stroke and from 41 male controls (July 2003-April 2007). RNA was isolated from blood and was processed using Affymetrix Human U133 Plus 2.0 expression arrays (Affymetrix Inc., Santa Clara, California). Y chromosome genes differentially expressed between male patients with stroke and male control subjects were identified using an ANCOVA adjusted for age and batch. A P < 0.05 and a fold change >1.2 were considered significant.
Seven genes on the Y chromosome were differentially expressed in males with ischemic stroke compared with controls. Five of these genes (VAMP7, CSF2RA, SPRY3, DHRSX, and PLCXD1) are located on pseudoautosomal regions of the human Y chromosome. The other 2 genes (EIF1AY and DDX3Y) are located on the nonrecombining region of the human Y chromosome. The identified genes were associated with immunology, RNA metabolism, vesicle fusion, and angiogenesis.
Specific genes on the Y chromosome are differentially expressed in blood after ischemic stroke. These genes provide insight into potential molecular contributors to sex differences in ischemic stroke.
有研究表明，性别是缺血性中风危险因素、病因及预后的重要决定因素。然而，其背后的原因尚不清楚。Y染色体是男性特有的。Y染色体上在男性中表达且与中风相关的基因，可能是导致男性缺血性中风独特特征的重要遗传因素，这将有助于解释男女之间观察到的性别差异。
我们比较了缺血性中风男性患者与男性对照者的Y染色体基因表达情况。
于2003年7月至2007年4月期间，采集了40例缺血性中风男性患者在发病后3小时、5小时和24小时的血样，以及41例男性对照者的血样。从血液中分离出RNA，并使用Affymetrix Human U133 Plus 2.0表达芯片（Affymetrix公司，加利福尼亚州圣克拉拉）进行处理。采用协方差分析（ANCOVA）对年龄和批次进行校正，以确定中风男性患者与男性对照者之间差异表达的Y染色体基因。P<0.05且变化倍数>1.2被认为具有统计学意义。
与对照组相比，缺血性中风男性患者中有7个Y染色体基因差异表达。其中5个基因（VAMP7、CSF2RA、SPRY3、DHRSX和PLCXD1）位于人类Y染色体的假常染色体区域。另外2个基因（EIF1AY和DDX3Y）位于人类Y染色体的非重组区域。所鉴定的基因与免疫、RNA代谢、囊泡融合和血管生成相关。
缺血性中风后，Y染色体上的特定基因在血液中差异表达。这些基因有助于深入了解缺血性中风性别差异的潜在分子因素。
