Department of Neurology, the MIND Institute, University of California at Davis, Sacramento, CA, USA.
J Cereb Blood Flow Metab. 2012 May;32(5):780-91. doi: 10.1038/jcbfm.2011.179. Epub 2011 Dec 14.
This study examined the effects of gender on RNA expression after ischemic stroke (IS). RNA obtained from blood of IS patients (n=51; 153 samples at < or =3, 5, and 24 hours) and from matched controls (n=52) were processed on Affymetrix microarrays. Analyses of covariance for stroke versus control samples were performed separately for both genders and the regulated genes for females compared with males. In all, 242, 227, and 338 male-specific genes were regulated at < or =3, 5, and 24 hours after IS, respectively, of which 59 were regulated at all time points. Overall, 774, 3,437, and 571 female-specific stroke genes were regulated at < or =3, 5, and 24 hours, respectively, of which 152 were regulated at all time points. Male-specific stroke genes were associated with integrin, integrin-liked kinase, actin, tight junction, Wnt/β-catenin, RhoA, fibroblast growth factors (FGF), granzyme, and tumor necrosis factor receptor (TNFR)2 signaling. Female-specific stroke genes were associated with p53, high-mobility group box-1, hypoxia inducible factor (HIF)1α, interleukin (IL)1, IL6, IL12, IL18, acute-phase response, T-helper, macrophage, and estrogen signaling. Cell death signaling was overrepresented in both genders, although the molecules and pathways differed. Gender affects gene expression in the blood of IS patients, which likely implies gender differences in immune, inflammatory, and cell death responses to stroke.
本研究旨在探讨性别对缺血性中风（IS）后 RNA 表达的影响。从 IS 患者（n=51；3、5 和 24 小时时<或=153 个样本）和匹配对照者（n=52）的血液中提取 RNA，并用 Affymetrix 微阵列进行处理。分别对男性和女性中风与对照样本进行协方差分析，并比较女性与男性的调控基因。<或=3、5 和 24 小时后，分别有 242、227 和 338 个男性特异性基因发生调控，其中 59 个基因在所有时间点均发生调控。<或=3、5 和 24 小时后，分别有 774、3437 和 571 个女性特异性中风基因发生调控，其中 152 个基因在所有时间点均发生调控。男性特异性中风基因与整合素、整合素样激酶、肌动蛋白、紧密连接、Wnt/β-catenin、RhoA、成纤维细胞生长因子（FGF）、颗粒酶和肿瘤坏死因子受体（TNFR）2 信号有关。女性特异性中风基因与 p53、高迁移率族蛋白盒 1、缺氧诱导因子（HIF）1α、白细胞介素（IL）1、IL6、IL12、IL18、急性期反应、T 辅助细胞、巨噬细胞和雌激素信号有关。细胞死亡信号在两性中均过表达，尽管分子和途径不同。性别会影响 IS 患者血液中的基因表达，这可能意味着免疫、炎症和细胞死亡对中风的反应存在性别差异。
