School of Public Health, Southeast University, Nanjing 210009, Jiangsu, China.
Biomed Environ Sci. 2011 Dec;24(6):661-9. doi: 10.3967/0895-3988.2011.06.011.
This study aims to investigate and compare the toxic effects of four types of metal oxide (ZnO, TiO(2), SiO(2,) and Al(2)O(3)) nanoparticles with similar primary size (∼20 nm) on human fetal lung fibroblasts (HFL1) in vitro.
The HFL1 cells were exposed to the nanoparticles, and toxic effects were analyzed by using MTT assay, cellular morphology observation and Hoechst 33 258 staining.
The results show that the four types of metal oxide nanoparticles lead to cellular mitochondrial dysfunction, morphological modifications and apoptosis at the concentration range of 0.25-1.50 mg/mL and the toxic effects are obviously displayed in dose-dependent manner. ZnO is the most toxic nanomaterials followed by TiO(2), SiO(2), and Al(2)O(3) nanoparticles in a descending order.
The results highlight the differential cytotoxicity associated with exposure to ZnO, TiO(2), SiO(2), and Al(2)O(3) nanoparticles, and suggest an extreme attention to safety utilization of these nanomaterials.
本研究旨在探讨和比较四种类型的金属氧化物（ZnO、TiO₂、SiO₂ 和 Al₂O₃）纳米颗粒对体外人胎肺成纤维细胞（HFL1）的毒性作用，这些纳米颗粒的初级粒径（约 20nm）相似。
将 HFL1 细胞暴露于纳米颗粒中，通过 MTT 测定法、细胞形态观察和 Hoechst 33258 染色分析毒性作用。
结果表明，四种类型的金属氧化物纳米颗粒在 0.25-1.50mg/ml 的浓度范围内导致细胞线粒体功能障碍、形态改变和凋亡，且毒性作用呈明显的剂量依赖性。ZnO 是最具毒性的纳米材料，其次是 TiO₂、SiO₂ 和 Al₂O₃ 纳米颗粒，依次递减。
研究结果突出了暴露于 ZnO、TiO₂、SiO₂ 和 Al₂O₃ 纳米颗粒相关的细胞毒性差异，提示在这些纳米材料的安全应用方面应给予高度关注。
