[瘤内注射携带结核分枝杆菌热休克蛋白70和单纯疱疹病毒胸苷激酶基因的重组减毒沙门氏菌以抑制小鼠黑色素瘤生长]
[Intratumor injection of recombinant attenuated salmonella carrying Mycobacterium tuberculosis heat shock protein 70 and herpes simplex virus thymidine kinase genes to suppress murine melanoma growth].
作者信息
Zeng Shuguang, Liu Qicai, Wang Suwen, Peng Ximao, Zhang Jincai, Zhang Jiren
机构信息
Department of Oral and Maxillofacial Surgery, Southern Medical University, Guangzhou, China.
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2012 Jan;32(1):101-5.
OBJECTIVE
To study the effection of suppression murine melanoma growth by Intratumor injection of recombinant attenuated salmonella carrying heat shock protein 70 and herpes simplex virus thymidine kinase genes.
METHODS
Plasmids PCMV-mtHSP70-IRES-TK were electro-transferred into salmonella typhimurium SL7207 to construct recombinant salmonella typhimurium. In vivo, Recombinant bacteria were injected into the mouse melanoma and the antitumor effection was observed. The survival period was recorded and safety analysis for this vaccine in each group.
RESULTS
In vivo, the mtHSP70/HSV-tk recombinant bacteria can suppress tumor growth significantly and extend survival. After recombinant Salmonella, 10(9) CFU/mL, was administered as an intratumoral injection, No diarrhea were observed. During therapy, body weight did not change markedly.
CONCLUSION
Results of the animal experiment suggests intratumor injection of recombinant attenuated salmonella typhimurium containing mtHSP70 and HSV-tk genes, has targeting ability against B16 tumor cell and could significantly inhibit tumor growth .
目的
研究瘤内注射携带热休克蛋白70和单纯疱疹病毒胸苷激酶基因的重组减毒沙门氏菌对小鼠黑色素瘤生长的影响。
方法
将质粒PCMV-mtHSP70-IRES-TK电转入鼠伤寒沙门氏菌SL7207构建重组鼠伤寒沙门氏菌。在体内，将重组菌注射到小鼠黑色素瘤中，观察其抗肿瘤效果。记录生存期并对每组该疫苗进行安全性分析。
结果
在体内，mtHSP70/HSV-tk重组菌能显著抑制肿瘤生长并延长生存期。以10(9) CFU/mL的重组沙门氏菌进行瘤内注射后，未观察到腹泻。治疗期间，体重无明显变化。
结论
动物实验结果表明，瘤内注射含mtHSP70和HSV-tk基因的重组减毒鼠伤寒沙门氏菌对B16肿瘤细胞具有靶向作用，可显著抑制肿瘤生长。
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