Unit of Internal Medicine, Angiology and Arteriosclerosis Diseases, Department of Clinical and Experimental Medicine, University of Perugia, Hospital Santa Maria della Misericordia, Piazzale Menghini, 1, 06129 Perugia, Italy.
Nutr Metab Cardiovasc Dis. 2013 May;23(5):466-72. doi: 10.1016/j.numecd.2011.08.006. Epub 2012 Feb 24.
Ectopic artery calcification has been documented in women with postmenopausal osteoporosis, in whom an imbalance in the number of circulating osteoprogenitor cells (OPCs) has been identified. Circulating OPCs form calcified nodules in vitro; however, it remains unknown whether an association exists between the number of circulating OPCs and aortic calcifications. We investigated the relationship between OPCs and aortic calcifications in women with postmenopausal osteoporosis.
The number of circulating OPCs was quantified by FACS analysis in 50 osteoporotic postmenopausal women. OPCs were defined as CD15-/alkaline-phosphatase(AP)+ cells coexpressing or not CD34. Participants underwent measurement of markers of bone metabolism, bone mineral density and abdominal aortic calcium (AAC) by 64-slice computed tomography. Patients with AAC were older, had lower 25(OH)vitamin D levels and higher circulating CD15-/AP+/CD34- cells than those without AAC. Significant correlates of AAC included age (rho = 0.38 p = 0.006), calcium (rho = 0.35 p = 0.01), 25(OH)vitamin D (rho = -0.31, p = 0.03) and the number of CD15-/AP+/CD34- cells (rho = 0.55 p < 0.001). In regression analyses, the log-transformed number of CD15-/AP+/CD34- cells was associated with the presence (OR = 6.45, 95% CI 1.03-40.1, p = 0.04) and severity (β = 0.43, p < 0.001) of AAC, independent of age, 25(OH)vitamin D, calcium and other potential confounders. Patients with low 25(OH)vitamin D and high CD15-/AP+/CD34- cells had higher median AAC than other patients (1927/μL, 862-2714/μL vs 147/μL, 0-1665/μL, p = 0.003).
In women with postmenopausal osteoporosis, the number of circulating CD15-/AP+/CD34- cells is significantly associated with increased aortic calcifications, that appear to be correlated also with reduced 25(OH)vitamin D levels.
绝经后骨质疏松症妇女中已发现异位动脉钙化，其中已鉴定出循环成骨祖细胞（OPC）数量失衡。循环 OPC 在体外形成钙化结节；然而，OPC 数量与主动脉钙化之间是否存在关联尚不清楚。我们研究了绝经后骨质疏松症妇女中 OPC 与主动脉钙化之间的关系。
通过流式细胞术分析定量测定 50 例骨质疏松绝经后妇女的循环 OPC 数量。OPC 被定义为 CD15-/碱性磷酸酶（AP）+细胞，其共同表达或不表达 CD34。参与者接受骨代谢标志物、骨矿物质密度和腹部主动脉钙（AAC）的 64 层计算机断层扫描测量。与无 AAC 患者相比，AAC 患者年龄较大，25（OH）维生素 D 水平较低，循环 CD15-/AP+/CD34-细胞较高。AAC 的显著相关因素包括年龄（rho=0.38，p=0.006）、钙（rho=0.35，p=0.01）、25（OH）维生素 D（rho=-0.31，p=0.03）和 CD15-/AP+/CD34-细胞数量（rho=0.55，p<0.001）。在回归分析中，对数转换的 CD15-/AP+/CD34-细胞数量与 AAC 的存在（OR=6.45，95%CI 1.03-40.1，p=0.04）和严重程度（β=0.43，p<0.001）相关，独立于年龄、25（OH）维生素 D、钙和其他潜在的混杂因素。25（OH）维生素 D 水平低和 CD15-/AP+/CD34-细胞高的患者的中位数 AAC 高于其他患者（1927/μL，862-2714/μL 与 147/μL，0-1665/μL，p=0.003）。
在绝经后骨质疏松症妇女中，循环 CD15-/AP+/CD34-细胞的数量与主动脉钙化的增加显著相关，而这种钙化似乎与 25（OH）维生素 D 水平降低有关。
