Unit of Internal Medicine, Angiology and Arteriosclerosis Diseases, Department of Clinical and Experimental Medicine, University of Perugia, Hospital Santa Maria della Misericordia, Piazzale Menghini 1, Perugia, Italy.
Nutr Metab Cardiovasc Dis. 2011 Sep;21(9):636-42. doi: 10.1016/j.numecd.2010.01.015. Epub 2010 May 31.
An increased number of circulating osteoprogenitor cells (OPCs) expressing bone-related proteins and the stem cell marker CD34 have been identified in women with postmenopausal osteoporosis, who also have stiffer arteries than nonosteoporotic subjects. We investigated whether an increased number of circulating OPCs underlies the association of osteoporosis with arterial stiffness.
The number of circulating OPCs was quantified by FACS analysis in 120 postmenopausal women with or without osteoporosis. OPCs were defined as CD34+/alkaline phosphatase(AP)+ or CD34+/osteocalcin(OCN)+ cells. Participants underwent cardiovascular risk factor assessment, measurement of bone mineral density (BMD), and aortic pulse wave velocity (aPWV) as a measure of arterial stiffness. Osteoporotic women had higher aPWV (9.8 ± 2.8 vs 8.5 ± 1.9 m/s, p = 0.005) and levels of CD34+/AP+ and CD34+/OCN+ cells than nonosteoporotic controls [1045 n/mL (487-2300) vs 510 n/mL (202-940), p < 0.001; 2415 n/mL (1225-8090) vs 1395 n/mL (207-2220), p < 0.001]. aPWV was associated with log-CD34+/AP+ (r = 0.27, p = 0.003), log-CD34+/OCN+ cells (r = 0.38, p < 0.001). In stepwise regression analysis CD34+/OCN+ cells, age, systolic blood pressure and heart rate were significant predictors of aPWV (Model R = 0.62, p < 0.001), independent of cardiovascular risk factors, parathyroid hormone levels and osteoporotic status.
In women with postmenopausal osteoporosis an increased availability of circulating osteoprogenitor cells has a detrimental influence on arterial compliance, which may in part explain the association between osteoporosis and arterial stiffening.
绝经后骨质疏松症患者的循环成骨祖细胞（OPCs）数量增加，这些细胞表达与骨相关的蛋白和干细胞标志物 CD34，且这些患者的动脉僵硬度也高于非骨质疏松症患者。我们研究了循环 OPC 数量的增加是否是骨质疏松症与动脉僵硬度相关的基础。
通过流式细胞术分析，我们对 120 例绝经后骨质疏松症或非骨质疏松症患者的循环 OPC 数量进行了定量检测。OPCs 被定义为 CD34+/碱性磷酸酶（AP）+或 CD34+/骨钙素（OCN）+细胞。参与者接受了心血管危险因素评估、骨密度（BMD）测量和脉搏波速度（aPWV）测量，以评估动脉僵硬度。与非骨质疏松症对照组相比，骨质疏松症患者的 aPWV 更高（9.8±2.8 比 8.5±1.9 m/s，p=0.005），且 CD34+/AP+和 CD34+/OCN+细胞的水平也更高[1045 n/mL（487-2300）比 510 n/mL（202-940），p<0.001；2415 n/mL（1225-8090）比 1395 n/mL（207-2220），p<0.001]。aPWV 与 log-CD34+/AP+（r=0.27，p=0.003）和 log-CD34+/OCN+细胞（r=0.38，p<0.001）呈正相关。逐步回归分析显示，CD34+/OCN+细胞、年龄、收缩压和心率是 aPWV 的显著预测因子（模型 R=0.62，p<0.001），独立于心血管危险因素、甲状旁腺激素水平和骨质疏松症状态。
绝经后骨质疏松症患者的循环成骨祖细胞的可用性增加，对动脉顺应性产生了不良影响，这可能部分解释了骨质疏松症与动脉僵硬度之间的关联。
