一种快速微量板血清杀菌测定法，用于测定血清补体介导的杀嗜血支原体作用。

A rapid microtiter plate serum bactericidal assay method for determining serum complement-mediated killing of Mannheimia haemolytica.

机构信息

Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Stillwater, Oklahoma 74078, USA.

出版信息

J Microbiol Methods. 2012 May;89(2):99-101. doi: 10.1016/j.mimet.2012.02.005. Epub 2012 Feb 16.

DOI:10.1016/j.mimet.2012.02.005

Abstract

In this study, we describe a rapid microtiter serum bactericidal assay (RMSBA) that can be used to measure the functionality of immune sera. It quantifies bactericidal activity of immune sera in the presence of complement against a homologous bacterium, M. haemolytica in this case. There is high correlation between data from RMSBA and standard complement-mediated bacterial killing assay (r=0.756; p<0.0001). The RMSBA activity of sera can be generated in less than 5 h instead of overnight incubation. RMSBA costs substantially less in terms of time, labor, and resources and is highly reproducible.

摘要

在这项研究中，我们描述了一种快速微量血清杀菌测定法（RMSBA），可用于测量免疫血清的功能。它定量了在补体存在的情况下针对同源细菌（在此情况下为 M. haemolytica）的免疫血清的杀菌活性。RMSBA 数据与标准补体介导的细菌杀伤测定法之间具有高度相关性（r=0.756；p<0.0001）。与过夜孵育相比，血清的 RMSBA 活性可以在不到 5 小时内产生。RMSBA 在时间、劳动力和资源方面的成本大大降低，并且具有高度可重复性。