一种用于研究抗体介导的细菌杀伤作用的快速微量方法，特别适用于绵羊溶血巴斯德菌感染的研究。
A rapid micro-method for the study of antibody-mediated killing of bacteria, with specific application to infection of sheep with Pasteurella haemolytica.
作者信息
Sutherland A D
机构信息
Moredun Research Institute, Edinburgh, Gt. Britain.
出版信息
Vet Microbiol. 1988 Mar;16(3):263-71. doi: 10.1016/0378-1135(88)90030-2.
A micro-titration plate bactericidal assay was developed to measure complement-dependent antibody-mediated killing of Pasteurella haemolytica. Sera and lung washings from specific pathogen-free (SPF) lambs convalescent from a challenge with live, virulent P. haemolytica were bactericidal in the presence of complement. Similar samples from naive SPF lambs had no such activity. Purified IgG derived from a convalescent lamb serum was as bactericidal as the whole serum. Absorption of convalescent serum with lipopolysaccharide from P. haemolytica abolished bactericidal activity, suggesting that this antigen may be a target for antibody in the bactericidal complex.
开发了一种微量滴定板杀菌试验，用于测定补体依赖的抗体介导的溶血巴斯德菌杀伤作用。来自经活的、有毒力的溶血巴斯德菌攻毒后恢复的无特定病原体（SPF）羔羊的血清和肺洗液，在补体存在的情况下具有杀菌作用。来自未接触过病原体的SPF羔羊的类似样本则没有这种活性。从一只恢复羔羊血清中提取的纯化IgG与全血清一样具有杀菌作用。用溶血巴斯德菌的脂多糖吸收恢复期血清可消除杀菌活性，这表明该抗原可能是杀菌复合物中抗体的靶标。
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