INT "G. Pascale", CROM-Oncology Research Centre of Mercogliano, Mercogliano, Italy.
Clin Biochem. 2012 May;45(7-8):525-9. doi: 10.1016/j.clinbiochem.2012.02.004. Epub 2012 Feb 16.
This study was aimed at searching noninvasive markers of the transition from mild to severe fibrosis stage in HCV patients undergoing hepatic fibrosis.
Thirty-three patients affected by chronic HCV vs. twenty healthy donors were evaluated for the serum levels of several circulating matrix metalloproteinases (MMPs), TRAIL and β-NGF by multiplex biometric ELISA based immunoassay and anti- and pro-oxidant status (d-ROMs, BAP and NO) using a Diacron automated method.
HCV patients displayed increased expression levels of MMP-8, MMP-9, TRAIL and β-NGF, and an imbalance between pro- and antioxidant status, that contribute to liver fibrosis.
Since the determination of these parameters represents a reliable and easily applicable method, these parameters are suggested as serum surrogate markers for HCV patients in the routine clinical practice.
本研究旨在寻找丙型肝炎患者从轻度纤维化向严重纤维化阶段转变的非侵入性标志物。
对 33 名慢性丙型肝炎患者和 20 名健康供体进行了基于多重生物计量 ELISA 的免疫分析，以评估几种循环基质金属蛋白酶（MMPs）、TRAIL 和 β-NGF 的血清水平，并使用 Diacron 自动化方法评估抗和促氧化剂状态（d-ROMs、BAP 和 NO）。
丙型肝炎患者 MMP-8、MMP-9、TRAIL 和 β-NGF 的表达水平升高，以及促氧化剂和抗氧化剂状态之间的失衡，这有助于肝纤维化的发生。
由于这些参数的测定是一种可靠且易于应用的方法，因此建议将这些参数作为丙型肝炎患者常规临床实践中的血清替代标志物。
