Institute of Chemical Technology and Engineering, Poznan University of Technology, Pl. M. Skłodowskiej-Curie 2, 60-965 Poznań, Poland.
Bioresour Technol. 2012 May;111:328-35. doi: 10.1016/j.biortech.2012.01.181. Epub 2012 Feb 9.
Bacterial utilization of rhamnolipids during biosurfactant-supplemented biodegradation of diesel and B20 (20% biodiesel and 80% diesel v/v) fuels was evaluated under conditions with full aeration or with nitrate and nitrite as electron acceptors. Rhamnolipid-induced changes in community dynamics were assessed by employing real-time PCR and the ddCt method for relative quantification. The experiments with rhamnolipids at 150 mg/l, approx. double critical micelle concentration (CMC) and diesel oil confirmed that rhamnolipids were readily degraded by a soil-isolated consortium of hydrocarbon degraders in all samples, under both aerobic and nitrate-reducing conditions. The presence of rhamnolipids increased the dissipation rates for B20 constituents under aerobic conditions, but did not influence the biodegradation rate of pure diesel. No effect was observed under nitrate-reducing conditions. The biodegradation of rhamnolipids did not favor the growth of any specific consortium member, which proved that the employed biosurfactant did not interfere with the microbial equilibrium during diesel/biodiesel biodegradation.
在有氧条件或硝酸盐和亚硝酸盐作为电子受体的条件下，评估了生物表面活性剂补充生物降解柴油和 B20（20%生物柴油和 80%柴油 v/v）燃料过程中细菌对鼠李糖脂的利用情况。通过实时 PCR 和 ddCt 方法进行相对定量，评估了鼠李糖脂对群落动态的影响。在 150mg/l、约两倍临界胶束浓度（CMC）和柴油的实验中，证实了在有氧和硝酸盐还原条件下，鼠李糖脂很容易被烃降解菌的土壤分离物共混物降解。在有氧条件下，鼠李糖脂的存在增加了 B20 成分的耗散速率，但不影响纯柴油的生物降解率。在硝酸盐还原条件下没有观察到这种影响。鼠李糖脂的生物降解并没有促进任何特定共混物成员的生长，这证明所使用的生物表面活性剂在柴油/生物柴油生物降解过程中不会干扰微生物平衡。
