Department of Zoology and Animal Physiology, Faculty of Science, University of Buea, P.O. Box 63 Buea, Cameroon. taiwe
J Ethnopharmacol. 2012 May 7;141(1):234-41. doi: 10.1016/j.jep.2012.02.024. Epub 2012 Feb 17.
The leaves of Crassocephalum bauchiense have long been used in traditional Cameroonian medicine for the treatment of epilepsy, pain, inflammatory disorders, arthritis and intestinal pain.
In this study, we attempted to identify the possible antinociceptive action of the aqueous extract and the alkaloid fraction prepared from the leaves of Crassocephalum baucheiense.
Using acetic acid induced abdominal constrictions, formalin-, capsaisin- and glutamate-induced nociception, and hot plate assay procedures, the antinociceptive effects of the aqueous extract and the alkaloid fraction was assessed after oral administration in mice. Morphine sulfate was used as reference analgesic agent. Mice were submitted to the rota-rod task and open-field test in order to assess any non-specific muscle-relaxant or sedative effects of the extracts of Crassocephalum bauchiense. Male and female Swiss mice were used to assess acute toxicity of these extracts.
The aqueous extract and the alkaloid fraction of Crassocephalum bauchiense produced a significant antinociceptive effects in the acetic acid, formalin, glutamate, capsaicin and hot plate tests. These antinociceptive effects of Crassocephalum bauchiense were significantly attenuated by pretreatment with naloxone. The extracts of Crassocephalum bauchiense did not alter the locomotion of animals in the open-field or rotarod tests, which suggest a lack of a central depressant effect. The animals did not exhibit any acute toxicity to the aqueous extract and the alkaloid fraction, so it was not possible to calculate the LD(50).
The results confirm the popular use of Crassocephalum bauchiense as an antinociceptive, and contribute to the pharmacological knowledge of this species because it was shown that the aqueous extract and the alkaloid fraction of Crassocephalum bauchiense produced dose related antinociception in models of chemical and thermal nociception through mechanisms that involve an interaction with opioidergic pathway.
Crassocephalum bauchiense 的叶子在传统喀麦隆医学中一直被用于治疗癫痫、疼痛、炎症性疾病、关节炎和肠道疼痛。
在这项研究中，我们试图确定从 Crassocephalum baucheiense 叶子中提取的水提物和生物碱部分的可能的镇痛作用。
使用醋酸诱导的腹部收缩、福尔马林、辣椒素和谷氨酸诱导的疼痛、热板试验程序，在小鼠口服后评估水提物和生物碱部分的镇痛作用。硫酸吗啡用作参考镇痛剂。为了评估 Crassocephalum bauchiense 提取物是否具有非特异性肌肉松弛或镇静作用，将小鼠进行旋转棒任务和旷场试验。使用雄性和雌性瑞士小鼠评估这些提取物的急性毒性。
Crassocephalum bauchiense 的水提物和生物碱部分在醋酸、福尔马林、谷氨酸、辣椒素和热板试验中产生了显著的镇痛作用。Crassocephalum bauchiense 的这些镇痛作用被纳洛酮预处理显著减弱。Crassocephalum bauchiense 的提取物在旷场或旋转棒试验中不改变动物的运动，这表明没有中枢抑制剂的作用。动物对水提物和生物碱部分没有表现出任何急性毒性，因此无法计算 LD(50)。
结果证实了 Crassocephalum bauchiense 作为一种镇痛剂的民间用途，并为该物种的药理学知识做出了贡献，因为已经表明 Crassocephalum bauchiense 的水提物和生物碱部分通过涉及与阿片样物质途径相互作用的机制，在化学和热痛觉模型中产生了剂量相关的镇痛作用。
