Department of Orthopaedics, Institute of Clinical Sciences, University of Gothenburg, Gothenburg, Sweden.
Spine (Phila Pa 1976). 2012 Aug 1;37(17):1440-7. doi: 10.1097/BRS.0b013e31824ff87d.
Descriptive experimental study.
The aim of this study was to investigate the effect of exercise on cell proliferation in different areas of the intervertebral disc (IVD) and recruitment of cells possibly active in regeneration of normal rat lumbar IVDs.
Little is known about the effects of physical exercise on lumbar IVD tissue. Recently, stem cell niches in the perichondrium area of the IVD were identified and cells in these niches have been suggested to be involved in the normal regeneration of the IVD.
Thirty Sprague-Dawley rats were exposed to 5-bromo-2-deoxyuridine (BrdU) diluted in the drinking water during 14 days. Fifteen rats ran on a treadmill daily for 50 min/d, 5 d/wk (exercise group), and 15 nonexercised rats served as controls. Immunohistochemical analyses (anti-BrdU antibody) were performed at 9, 14, 28, 56, and 105 days after the start of the exercise protocol. BrdU positive cells were counted in the stem cell niche area, the peripheral region of epiphyseal cartilage area, and the annulus fibrous outer and inner areas. Data were analyzed by 2-way analysis of variance (significance level; P < 0.05).
The BrdU positive cell numbers in the stem cell niche and annulus fibrous outer regions were increased in discs from the exercising group on days 14 (P < 0.01) and 105 (P < 0.05) and at day 14 (P < 0.01) in the peripheral epiphyseal cartilage region compared with controls.
Physical exercise was shown to have positive effects on cell proliferation in IVDs, with involvement of various disc regions, indicating a differential response by disc tissue to exercise depending on anatomical location and tissue characteristics.
描述性实验研究。
本研究旨在探讨运动对椎间盘（IVD）不同区域细胞增殖的影响，并招募可能参与正常大鼠腰椎 IVD 再生的细胞。
对于体育锻炼对腰椎 IVD 组织的影响知之甚少。最近，在 IVD 的软骨膜区域确定了干细胞龛，并且这些龛中的细胞被认为参与了 IVD 的正常再生。
30 只 Sprague-Dawley 大鼠在饮用水中摄入 5-溴-2-脱氧尿苷（BrdU）14 天。15 只大鼠每天在跑步机上运动 50 分钟/天，每周 5 天（运动组），15 只不运动的大鼠作为对照组。在运动方案开始后 9、14、28、56 和 105 天进行免疫组织化学分析（抗 BrdU 抗体）。在干细胞龛区、骺软骨区外周区和纤维环纤维外层和内层计数 BrdU 阳性细胞。数据通过双因素方差分析进行分析（显著性水平；P < 0.05）。
运动组椎间盘的干细胞龛和纤维环外层区域的 BrdU 阳性细胞数量在第 14 天（P < 0.01）和第 105 天（P < 0.05）以及第 14 天（P < 0.01）时均高于对照组。骺软骨区外周区。
运动对 IVD 细胞增殖有积极影响，涉及多个椎间盘区域，表明椎间盘组织对运动的反应因解剖位置和组织特征而异。
