Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, United Kingdom.
Genetics. 2012 May;191(1):119-31. doi: 10.1534/genetics.111.137976. Epub 2012 Feb 23.
In Neurospora crassa, the interactions between products of the frequency (frq), frequency-interacting RNA helicase (frh), white collar-1 (wc-1), and white collar-2 (wc-2) genes establish a molecular circadian clockwork, called the FRQ-WC-Oscillator (FWO), which is required for the generation of molecular and overt circadian rhythmicity. In strains carrying nonfunctional frq alleles, circadian rhythms in asexual spore development (conidiation) are abolished in constant conditions, yet conidiation remains rhythmic in temperature cycles. Certain characteristics of these temperature-synchronized rhythms have been attributed to the activity of a FRQ-less oscillator (FLO). The molecular components of this FLO are as yet unknown. To test whether the FLO depends on other circadian clock components, we created a strain that carries deletions in the frq, wc-1, wc-2, and vivid (vvd) genes. Conidiation in this ΔFWO strain was still synchronized to cyclic temperature programs, but temperature-induced rhythmicity was distinct from that seen in single frq knockout strains. These results and other evidence presented indicate that components of the FWO are part of the temperature-induced FLO.
在粗糙脉孢菌中，频率（frq）、频率相互作用 RNA 解旋酶（frh）、白颈-1（wc-1）和白颈-2（wc-2）基因产物之间的相互作用建立了一个分子生物钟，称为 FRQ-WC-Oscillator（FWO），它是产生分子和显性昼夜节律所必需的。在携带非功能 frq 等位基因的菌株中，无性孢子发育（分生孢子形成）的昼夜节律在恒定条件下被消除，但在温度循环中分生孢子形成仍然有节奏。这些温度同步节律的某些特征归因于无 FRQ 振荡器（FLO）的活性。这个 FLO 的分子组成部分目前尚不清楚。为了测试 FLO 是否依赖于其他生物钟成分，我们创建了一个在 frq、wc-1、wc-2 和 vivid（vvd）基因中缺失的菌株。这个 ΔFWO 菌株的分生孢子形成仍然与周期性温度程序同步，但温度诱导的节律性与在单个 frq 敲除菌株中观察到的节律性不同。这些结果和其他证据表明，FWO 的成分是温度诱导的 FLO 的一部分。
