Department of Chemical and Materials Engineering, Tunghai University, Taichung, Taiwan.
Bioprocess Biosyst Eng. 2012 Oct;35(8):1251-8. doi: 10.1007/s00449-012-0712-6. Epub 2012 Feb 26.
Antrodia cinnamomea has recently become a well-known medicinal mushroom in Taiwan. Bioactive compounds found in A. cinnamomea include: polysaccharide, sesquiterpene lactone, steroids and triterpenoids. The aim of this study was to evaluate the feasibility of adding citrus peel extract to enhance the formation of bioactive metabolites in the submerged culture of A. cinnamomea. With the exception of grapefruit, citrus peel extracts tested were proved to be beneficial to mycelial growth and to the production of intracellular polysaccharide. Lemon was the most effective for enhancing bioactive metabolite production. With an addition of 2% (v/v), the mycelium biomass concentration and intracellular polysaccharide content rose from 11.96 g DW/L of the control and 123.6 mg/g DW to 21.96 g DW/L and 230.8 mg/g DW, respectively, on day 8. The production of triterpenoids also increased from 86.7 to 282.9 mg/L. Moreover, this study also demonstrates that although the addition of peel extract could cause the lengthening of the exponential phase and reduce the specific growth rate, the production rate of biomass, intracellular polysaccharide and triterpenoids was still enhanced significantly.
樟芝近年来已成为台湾知名的药用蕈类。在樟芝中发现的生物活性化合物包括：多糖、倍半萜内酯、类固醇和三萜。本研究旨在评估添加柑橘皮提取物以增强樟芝液体深层培养中生物活性代谢物形成的可行性。除了葡萄柚外，所测试的柑橘皮提取物均被证明有利于菌丝体生长和细胞内多糖的产生。柠檬对增强生物活性代谢产物的产生最有效。添加 2%（v/v）时，第 8 天菌丝体生物量浓度和细胞内多糖含量分别从对照的 11.96 g DW/L 和 123.6 mg/g DW 增加到 21.96 g DW/L 和 230.8 mg/g DW，三萜的产量也从 86.7 增加到 282.9 mg/L。此外，本研究还表明，尽管添加果皮提取物会导致指数生长期延长并降低比生长速率，但生物量、细胞内多糖和三萜的生产速率仍显著提高。
