Department of Forestry, National Chung-Hsing University, Taichung, Taiwan.
J Agric Food Chem. 2011 Jul 27;59(14):7626-35. doi: 10.1021/jf201632w. Epub 2011 Jun 21.
Antrodia cinnamomea is a precious edible fungus endemic to Taiwan that has long been used as a folk remedy for health promotion and for treating various diseases. In this study, an index of 13 representative metabolites from the ethanol extract of A. cinnamomea fruiting body was established for use in quality evaluation. Most of the index compounds selected, particularly the ergostane-type triterpenoids and polyacetylenes, possess good anti-inflammation activity. A comparison of the metabolite profiles of different ethanol extracts from A. cinnamomea strains showed silmilar metabolites when the strains were grown on the original host wood (Cinnamomum kanehirai) and harvested after the same culture time period (9 months). Furthermore, the amounts of typical ergostane-type triterpenoids in A. cinnamomea increased with culture age. Culture substrates also influenced metabolite synthesis; with the same culture age, A. cinnamomea grown on the original host wood produced a richer array of metabolites than A. cinnamomea cultured on other wood species. We conclude that analysis of a fixed group of compounds including triterpenoids, benzolics, and polyacetylenes constitutes a suitable, reliable system to evaluate the quality of ethanol extract from A. cinnamomea fruiting bodies. The evaluation system established in this study may provide a platform for analysis of the products of A. cinnamomea.
樟芝是一种珍贵的可食用真菌，仅分布于中国台湾地区，长期以来一直被用作促进健康和治疗各种疾病的民间药物。在这项研究中，建立了一个由 13 种代表代谢物组成的指数，用于评估樟芝子实体乙醇提取物的质量。所选的大多数指标化合物，特别是麦角甾烷型三萜类化合物和多炔类化合物，具有良好的抗炎活性。比较不同樟芝菌株的乙醇提取物的代谢谱，当菌株在原始宿主木材（肉桂）上生长并在相同的培养时间（9 个月）后收获时，显示出相似的代谢物。此外，随着培养年龄的增加，樟芝中的典型麦角甾烷型三萜类化合物的含量增加。培养基质也会影响代谢物的合成；在相同的培养年龄下，在原始宿主木材上生长的樟芝比在其他木材上培养的樟芝产生更丰富的代谢物。我们得出结论，分析包括三萜类化合物、苯丙素类化合物和多炔类化合物在内的固定化合物群构成了评估樟芝子实体乙醇提取物质量的合适、可靠的系统。本研究中建立的评价体系可以为樟芝产品的分析提供一个平台。
