Department of Biotechnology, Indian Institute of Technology, Roorkee 247667, India.
Funct Integr Genomics. 2012 Jun;12(2):291-304. doi: 10.1007/s10142-012-0264-5. Epub 2012 Feb 25.
A T-DNA insertional mutant OsTEF1 of rice gives 60-80% reduced tillering, retarded growth of seminal roots, and sensitivity to salt stress compared to wild type Basmati 370. The insertion occurred in a gene encoding a transcription elongation factor homologous to yeast elf1, on chromosome 2 of rice. Detailed transcriptomic profiling of OsTEF1 revealed that mutation in the transcription elongation factor differentially regulates the expression of more than 100 genes with known function and finely regulates tillering process in rice by inducing the expression of cytochrome P450. Along with different transcription factors, several stress associated genes were also affected due to a single insertion. In silico analysis of the TEF1 protein showed high conservation among different organisms. This transcription elongation factor predicted to interact with other proteins that directly or indirectly positively regulate tillering in rice.
与野生型 Basmati 370 相比，水稻 T-DNA 插入突变体 OsTEF1 的分蘖减少了 60-80%，初生根生长受阻，对盐胁迫敏感。该插入发生在编码与酵母 elf1 同源的转录延伸因子的基因中，位于水稻的第 2 号染色体上。对 OsTEF1 的详细转录组分析表明，转录延伸因子的突变通过诱导细胞色素 P450 的表达，差异调节了 100 多个具有已知功能的基因的表达，并精细地调节了水稻的分蘖过程。除了不同的转录因子外，由于单个插入，几个与应激相关的基因也受到了影响。TEF1 蛋白的计算机分析表明，不同生物体之间具有高度的保守性。该转录延伸因子预测与其他直接或间接正向调节水稻分蘖的蛋白质相互作用。
