Ahmad Mushtaq
Visiting Scientist Plant Sciences, University of Nebraska, Lincoln, NE, United States.
Front Plant Sci. 2022 Oct 20;13:1002596. doi: 10.3389/fpls.2022.1002596. eCollection 2022.
More over half of the world's population depends on rice as a major food crop. Rice ( L.) is vulnerable to abiotic challenges including drought, cold, and salinity since it grown in semi-aquatic, tropical, or subtropical settings. Abiotic stress resistance has bred into rice plants since the earliest rice cultivation techniques. Prior to the discovery of the genome, abiotic stress-related genes were identified using forward genetic methods, and abiotic stress-tolerant lines have developed using traditional breeding methods. Dynamic transcriptome expression represents the degree of gene expression in a specific cell, tissue, or organ of an individual organism at a specific point in its growth and development. Transcriptomics can reveal the expression at the entire genome level during stressful conditions from the entire transcriptional level, which can be helpful in understanding the intricate regulatory network relating to the stress tolerance and adaptability of plants. Rice ( L.) gene families found comparatively using the reference genome sequences of other plant species, allowing for genome-wide identification. Transcriptomics gene expression profiling which have recently dominated by RNA-seq complements genomic techniques. The identification of numerous important qtl,s genes, promoter elements, transcription factors and miRNAs involved in rice response to abiotic stress was made possible by all of these genomic and transcriptomic techniques. The use of several genomes and transcriptome methodologies to comprehend rice (, L.) ability to withstand abiotic stress have been discussed in this review.
此外,世界上超过一半的人口依赖水稻作为主要粮食作物。水稻(Oryza sativa L.)易受非生物胁迫的影响,包括干旱、寒冷和盐碱化,因为它生长在半水生、热带或亚热带环境中。自最早的水稻种植技术以来,非生物胁迫抗性就已培育到水稻植株中。在发现基因组之前,使用正向遗传学方法鉴定与非生物胁迫相关的基因,并使用传统育种方法培育非生物胁迫耐受性品系。动态转录组表达代表个体生物体在其生长和发育的特定阶段,特定细胞、组织或器官中的基因表达程度。转录组学可以从整个转录水平揭示应激条件下全基因组水平的表达情况,这有助于理解与植物胁迫耐受性和适应性相关的复杂调控网络。通过比较其他植物物种的参考基因组序列发现水稻(Oryza sativa L.)基因家族,从而实现全基因组鉴定。最近以RNA测序为主导的转录组学基因表达谱分析补充了基因组技术。所有这些基因组和转录组技术使得鉴定参与水稻对非生物胁迫响应的众多重要QTL、基因、启动子元件、转录因子和miRNA成为可能。本综述讨论了使用多种基因组和转录组方法来理解水稻(Oryza sativa, L.)耐受非生物胁迫的能力。