Yang Shuhua, Zhang Ziwei, He Jianbin, Li Jinlong, Zhang Jiuli, Xing Houjuan, Xu Shiwen
College of Veterinary Medicine, Northeast Agricultural University, Number 59, Mucai Street, Harbin, 150030, People's Republic of China.
Biol Trace Elem Res. 2012 Jul;148(1):53-60. doi: 10.1007/s12011-012-9343-7. Epub 2012 Feb 25.
To investigate the toxicity of cadmium (Cd) on female reproduction in birds, this study was conducted to determine the changes in biochemical parameters of serum and ovary tissue caused by dietary cadmium in hens. Ninety 50-day-old hyline white hens were randomly divided into three groups (30 hens per group): a control group was fed with basal diet, a low dose group was fed with basal diet containing 140 mg/kg CdCl(2) and a high dose group was fed with basal diet containing 210 mg/kg CdCl2. After being treated with Cd for 20, 40 and 60 days, ovary and serum samples were collected and examined for Cd content, histological evaluations, malondialdehyde (MDA) content, glutathione peroxidase (GPx) content, activities of superoxide dismutase (SOD), nitric oxide (NO) content, nitric oxide synthase (NOS) activity, and serum estradiol and progestogen levels. The results showed that the content of Cd, MDA, NO and the activity of NOS in ovary and serum were increased (P < 0.05), while the level of GPx and the activity of SOD were decreased (P < 0.05) in low dose and high dose groups. A time- and dose-dependent correlation was observed between serum and ovary tissue cadmium levels. The number of apoptotic cells in the ovary was increased in the Cd treatment group (P < 0.05). Extensive damage was observed in the ovary. The level of estradiol and progestogen in the serum of low dose and high dose groups was decreased significantly (P < 0.05). It indicated that Cd exposure resulted in oxidative damage of hens' ovary tissue by altering antioxidant defense enzyme systems, lipid peroxidation, apoptosis and endocrine disturbance which may be possible underlying reproductive toxicity mechanisms induced by Cd.
为研究镉（Cd）对鸟类雌性生殖的毒性，本研究旨在确定日粮镉对母鸡血清和卵巢组织生化参数的影响。将90只50日龄海兰白母鸡随机分为三组（每组30只）：对照组饲喂基础日粮，低剂量组饲喂含140 mg/kg CdCl₂的基础日粮，高剂量组饲喂含210 mg/kg CdCl₂的基础日粮。用镉处理20、40和60天后，采集卵巢和血清样本，检测镉含量、组织学评估、丙二醛（MDA）含量、谷胱甘肽过氧化物酶（GPx）含量、超氧化物歧化酶（SOD）活性、一氧化氮（NO）含量、一氧化氮合酶（NOS）活性以及血清雌二醇和孕激素水平。结果显示，低剂量组和高剂量组卵巢及血清中镉、MDA、NO含量和NOS活性升高（P<0.05），而GPx水平和SOD活性降低（P<0.05）。血清和卵巢组织镉水平之间存在时间和剂量依赖性相关性。镉处理组卵巢中凋亡细胞数量增加（P<0.05），卵巢出现广泛损伤。低剂量组和高剂量组血清中雌二醇和孕激素水平显著降低（P<0.05）。这表明镉暴露通过改变抗氧化防御酶系统、脂质过氧化、细胞凋亡和内分泌紊乱导致母鸡卵巢组织氧化损伤，这可能是镉诱导生殖毒性的潜在机制。
