中枢神经系统神经元-神经胶质细胞共培养模型。

Central nervous system neuron-glia co-culture models.

作者信息

Skaper Stephen D, Facci Laura

机构信息

Department of Pharmacology and Anesthesiology, University of Padova, Padova, Italy.

出版信息

Methods Mol Biol. 2012;846:79-89. doi: 10.1007/978-1-61779-536-7_8.

DOI:10.1007/978-1-61779-536-7_8

PMID:22367803

Abstract

Glial cell activation plays an important role in the pathogenesis of various neurodegenerative disorders. This article presents a protocol for the preparation of cultures consisting of rat embryonic cortical neurons grown in the presence of cortical microglia, in which the glia are present in physical contact with the neurons or separated by a semi-permeable membrane barrier.

摘要

胶质细胞激活在各种神经退行性疾病的发病机制中起着重要作用。本文介绍了一种培养物制备方案，该培养物由在皮质小胶质细胞存在下生长的大鼠胚胎皮质神经元组成，其中胶质细胞与神经元物理接触或被半透膜屏障隔开。