Skaper Stephen D, Facci Laura
Department of Pharmacology and Anesthesiology, University of Padova, Padova, Italy.
Methods Mol Biol. 2012;846:79-89. doi: 10.1007/978-1-61779-536-7_8.
Glial cell activation plays an important role in the pathogenesis of various neurodegenerative disorders. This article presents a protocol for the preparation of cultures consisting of rat embryonic cortical neurons grown in the presence of cortical microglia, in which the glia are present in physical contact with the neurons or separated by a semi-permeable membrane barrier.
胶质细胞激活在各种神经退行性疾病的发病机制中起着重要作用。本文介绍了一种培养物制备方案，该培养物由在皮质小胶质细胞存在下生长的大鼠胚胎皮质神经元组成，其中胶质细胞与神经元物理接触或被半透膜屏障隔开。
