Latif Zahid, Sarker Satyajit D
Technology Strategy Board, Swindon, UK.
Methods Mol Biol. 2012;864:255-74. doi: 10.1007/978-1-61779-624-1_10.
Preparative HPLC (prep-HPLC) has emerged as a mainstay of natural products isolation and purification. The various modes available to date, e.g. normal phase, reversed-phase, size exclusion, and ion exchange, can be used to purify most classes of natural products. This chapter presents an overview of the different modes along with a practical guide as to how to purify a natural product using the most robust and widely used of the modes, namely reversed-phase prep-HPLC. Instrumentation set-up and detection methods, sample preparation, method development, and sample work up are also discussed.
制备型高效液相色谱（prep-HPLC）已成为天然产物分离与纯化的主要手段。目前可用的各种模式，如正相、反相、尺寸排阻和离子交换，可用于纯化大多数种类的天然产物。本章概述了不同的模式，并提供了一份实用指南，介绍如何使用最可靠且应用最广泛的模式，即反相制备型高效液相色谱来纯化天然产物。还讨论了仪器设置和检测方法、样品制备、方法开发以及样品后处理。
