McAlpine James B, Friesen J Brent, Pauli Guido F
Department of Medicinal Chemistry and Pharmacognosy, School of Pharmacy, University of Illinois at Chicago, Chicago, IL, USA.
Methods Mol Biol. 2012;864:221-54. doi: 10.1007/978-1-61779-624-1_9.
Countercurrent Chromatography (CCC) provides the natural product chemist with a high-resolution separatory method, which is uniquely applicable to sensitive (unstable) compounds and which allows virtually quantitative recovery of the load sample. Different instruments use different means of retaining a stationary liquid phase. The solvent system (SS) can be chosen to optimize the separatory power and the number of systems available is limitless. Several examples are provided to illustrate the power of the method and to guide the chemist in choice of an appropriate SS.
逆流色谱法（CCC）为天然产物化学家提供了一种高分辨率的分离方法，该方法特别适用于敏感（不稳定）化合物，并且几乎可以定量回收负载样品。不同的仪器采用不同的方式保留固定液相。可以选择溶剂系统（SS）以优化分离能力，并且可用的系统数量是无限的。提供了几个例子来说明该方法的功效，并指导化学家选择合适的溶剂系统。
