Glenn G M, Hayes D A, Armstrong R B
Miami Children's Hospital, FL 33155.
Res Commun Chem Pathol Pharmacol. 1990 Sep;69(3):353-6.
The purpose of this study was to determine the dose-response relationship between verapamil HCl and creatine kinase (CK) release from isolated rat slow (soleus, S) and fast (flexor digitorum profundus, F) skeletal muscles. Total CK activity in F muscle was approximately 2x greater than in S muscle. For both S and F muscles, CK release as a function of verapamil concentration in the incubation medium was linear (r = 0.94); the slope of the line for F muscle was 4.5x that of the S muscle. S and F muscles lost up to 38-44% of their total CK activity during the 2 hr incubation. The CK activity lost to the medium was 100% of the MM isoform. The mechanism of the enzyme release is not known.
本研究的目的是确定盐酸维拉帕米与离体大鼠慢肌（比目鱼肌，S）和快肌（趾长屈肌，F）骨骼肌中肌酸激酶（CK）释放之间的剂量反应关系。F肌中的总CK活性约比S肌中的高2倍。对于S肌和F肌，孵育培养基中CK释放量与维拉帕米浓度的关系呈线性（r = 0.94）；F肌的直线斜率是S肌的4.5倍。在2小时的孵育过程中，S肌和F肌损失了其总CK活性的38 - 44%。释放到培养基中的CK活性全部是MM同工型。酶释放的机制尚不清楚。
