Wang M, Wan L, Chen H, Liu X, Liu J
College of Life Science, Capital Normal University, Beijing, People's Republic of China.
Biotech Histochem. 2012 May;87(4):300-2. doi: 10.3109/10520295.2011.649784. Epub 2012 Feb 28.
To observe the dynamic behavior of callose of microspore mother cells during meiosis, we developed a convenient, rapid and efficient staining method using an improved carbol fuchsin/aniline blue solution. The stained microspore mother cells during meiosis showed yellowish green callose, red cytoplasm and dark red chromosomes when excited with blue light, which produced a contrasting image with a three-dimensional effect. When stained with only improved carbol fuchsin solution, the cells had red cytoplasm and chromosomes when excited with green light. The improved carbol fuchsin solution can be used to replace other more expensive DNA-specific dyes, such as DAPI and H33258, to reduce experimental costs.
为了观察小孢子母细胞减数分裂过程中胼胝质的动态行为，我们开发了一种简便、快速且高效的染色方法，使用改良的石炭酸品红/苯胺蓝溶液。减数分裂过程中经染色的小孢子母细胞在蓝光激发下呈现出黄绿色的胼胝质、红色的细胞质和深红色的染色体，产生具有三维效果的对比图像。当仅用改良的石炭酸品红溶液染色时，细胞在绿光激发下具有红色的细胞质和染色体。改良的石炭酸品红溶液可用于替代其他更昂贵的DNA特异性染料，如DAPI和H33258，以降低实验成本。
