Breath Research Institute, Austrian Academy of Sciences, Rathausplatz 4, A-6850 Dornbirn, Austria.
Physiol Meas. 2012 Mar;33(3):413-28. doi: 10.1088/0967-3334/33/3/413. Epub 2012 Feb 28.
This explorative study aims at characterizing the breath behavior of two prototypic volatile organic compounds, acetone and isoprene, during normal human sleep and to possibly relate changes in the respective concentration time courses to the underlying sleep architecture. For this purpose, six normal healthy volunteers (two females, four males, age 20-29 years) were monitored over two consecutive nights (the first one being an adaption night) by combining real-time proton-transfer-reaction mass spectrometry measurements from end-tidal exhalation segments with laboratory-based polysomnographic data. Breath acetone concentrations increased overnight in all measurements, with an average relative change by a factor of up to 4 (median 2.5). Nighttime concentration maxima were usually recorded 2-3 h before lights on. For breath isoprene, a nocturnal increase in baseline concentrations of about 74% was observed, with individual changes ranging from 36-110%. Isoprene profiles exhibited pronounced concentration peaks, which were highly specific for leg movements as scored by tibial electromyography. Furthermore, relative to a linear trend, baseline isoprene concentrations decreased during the transition from the NREM to the REM phase of a complete sleep cycle.
本探索性研究旨在描述两种典型挥发性有机化合物（丙酮和异戊二烯）在正常人类睡眠期间的呼吸行为，并可能将各自浓度时间曲线的变化与潜在的睡眠结构相关联。为此，我们在连续两个晚上（第一个晚上为适应期）对六名正常健康志愿者（两名女性，四名男性，年龄 20-29 岁）进行了监测，方法是将实时质子转移反应质谱测量从末端呼出段与实验室多导睡眠图数据相结合。在所有测量中，呼吸丙酮浓度在夜间逐渐升高，平均相对变化高达 4 倍（中位数为 2.5）。夜间浓度最大值通常在天亮前 2-3 小时记录。对于呼吸异戊二烯，观察到基线浓度约增加 74%，个体变化范围为 36-110%。异戊二烯谱显示出明显的浓度峰值，这些峰值与胫骨肌电图评分的腿部运动高度相关。此外，与线性趋势相比，基线异戊二烯浓度在完整睡眠周期的非快速眼动 (NREM) 向快速眼动 (REM) 阶段的过渡期间下降。
