Graduate School of Pharmaceutical Sciences, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8675, Japan.
Int J Pharm. 2012 May 10;427(2):365-71. doi: 10.1016/j.ijpharm.2012.02.020. Epub 2012 Feb 20.
Structural evaluation of probucol nanoparticles coground with polyvinylpyrrolidone K17 and sodium dodecyl sulfate for 90 min was performed by solid-state nuclear magnetic resonance (NMR) spectroscopy and atomic force microscopy (AFM) with force-distance curve analysis. The results of solid-state NMR indicated that the cogrinding changed crystalline probucol to amorphous form. The number-averaged mean heights of probucol particles in the ground mixture (GM) suspension were determined by AFM to be 6 and 15 nm for freshly prepared and 24h-stored samples, respectively. Nucleation and the subsequent crystal growth might have occurred after the GM was dispersed in water. The presence of probucol nanocrystals and agglomeration of the primary probucol nanoparticles were recognized by AFM force-distance curve analysis. AFM could be a promising tool to evaluate the structure of nanoparticles as well as their agglomeration behavior in aqueous media.
采用固态核磁共振（NMR）光谱和原子力显微镜（AFM）结合力-距离曲线分析对聚维酮 K17 和十二烷基硫酸钠共研磨 90min 的普罗布考纳米粒子进行结构评价。固态 NMR 的结果表明，共研磨将结晶普罗布考转化为无定形形式。原子力显微镜测定新鲜制备和 24 小时储存的共研磨混合物（GM）悬浮液中普罗布考颗粒的数均平均高度分别为 6nm 和 15nm。GM 在水中分散后可能发生成核和随后的晶体生长。原子力显微镜力-距离曲线分析表明存在普罗布考纳米晶体和初级普罗布考纳米粒子的团聚。原子力显微镜可以成为评估纳米颗粒结构及其在水介质中团聚行为的有前途的工具。
